Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 4 of 4) - Dataset (ID:20277)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein 1 Detected (647) Protein 2 Detected (647) Protein 3 Detected (647) Protein 4 Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib (+)-JQ1 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 133.91 NGFR rabbit IgG(H+L) / donkey NGFR 998.17
WM115-P3
G3
A-375 Vemurafenib (+)-JQ1 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 86.41 NGFR rabbit IgG(H+L) / donkey NGFR 79.59
A375-P3
G6
A-375 Vemurafenib (+)-JQ1 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 98.60 NGFR rabbit IgG(H+L) / donkey NGFR 146.35
A375-P3
G5
A-375 Vemurafenib (+)-JQ1 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 119.57 NGFR rabbit IgG(H+L) / donkey NGFR 119.71
A375-P3
G4
A-375 Vemurafenib (+)-JQ1 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 193.38 NGFR rabbit IgG(H+L) / donkey NGFR 97.06
A375-P3
G3
A-375 Vemurafenib (+)-JQ1 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 374.84 NGFR rabbit IgG(H+L) / donkey NGFR 191.92
A375-P3
G2
A-375 Vemurafenib (+)-JQ1 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 46.97 NGFR rabbit IgG(H+L) / donkey NGFR 88.97
A375-P3
F6
A-375 Vemurafenib (+)-JQ1 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 79.95 NGFR rabbit IgG(H+L) / donkey NGFR 158.37
A375-P3
F5
A-375 Vemurafenib (+)-JQ1 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 141.85 NGFR rabbit IgG(H+L) / donkey NGFR 125.72
A375-P3
F4
A-375 Vemurafenib (+)-JQ1 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 206.48 NGFR rabbit IgG(H+L) / donkey NGFR 104.55
A375-P3
F3
COLO 858 Vemurafenib (+)-JQ1 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 275.55 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 331.66
Colo858-P2
F6
COLO 858 Vemurafenib (+)-JQ1 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 362.01 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 74.07
Colo858-P1
F4
COLO 858 Vemurafenib (+)-JQ1 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 413.21 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 3537.67
Colo858-P4
F2
COLO 858 Vemurafenib (+)-JQ1 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 247.74 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1599.29
Colo858-P4
F3
COLO 858 Vemurafenib (+)-JQ1 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 204.72 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 916.68
Colo858-P4
F4
COLO 858 Vemurafenib (+)-JQ1 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 200.09 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 673.63
Colo858-P4
F5
COLO 858 Vemurafenib (+)-JQ1 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 171.18 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 671.08
Colo858-P4
F6
COLO 858 Vemurafenib (+)-JQ1 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 389.55 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 109.83
Colo858-P1
F3
WM-115 Vemurafenib (+)-JQ1 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 200.27 NGFR rabbit IgG(H+L) / donkey NGFR 1110.47
WM115-P3
F2
WM-115 Vemurafenib (+)-JQ1 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 168.93 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1239.42
WM115-P4
F2
WM-115 Vemurafenib (+)-JQ1 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 117.04 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1192.56
WM115-P4
G2
COLO 858 Vemurafenib (+)-JQ1 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 331.50 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 300.70
Colo858-P2
F5
COLO 858 Vemurafenib (+)-JQ1 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 386.59 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 3300.11
Colo858-P4
G2
COLO 858 Vemurafenib (+)-JQ1 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 243.32 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1522.29
Colo858-P4
G3
COLO 858 Vemurafenib (+)-JQ1 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 198.10 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 692.15
Colo858-P4
G4