Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 4 of 4) - Dataset (ID:20277)

 Full screen view
Filter results: >>>>
Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein 1 Detected (647) Protein 2 Detected (647) Protein 3 Detected (647) Protein 4 Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib Belinostat 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 72.12 NGFR rabbit IgG(H+L) / donkey NGFR 770.43
WM115-P3
E4
WM-115 Vemurafenib Belinostat 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 32.44 NGFR rabbit IgG(H+L) / donkey NGFR 724.88
WM115-P3
E5
WM-115 Vemurafenib Belinostat 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 11.76 NGFR rabbit IgG(H+L) / donkey NGFR 666.72
WM115-P3
E6
WM-115 Vemurafenib I-BET 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 111.82 NGFR rabbit IgG(H+L) / donkey NGFR 866.72
WM115-P3
E7
WM-115 Vemurafenib I-BET 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 107.98 NGFR rabbit IgG(H+L) / donkey NGFR 918.12
WM115-P3
E8
WM-115 Vemurafenib I-BET 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 51.30 NGFR rabbit IgG(H+L) / donkey NGFR 1033.29
WM115-P3
E9
WM-115 Vemurafenib I-BET151 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 21.85 NGFR rabbit IgG(H+L) / donkey NGFR 1033.43
WM115-P3
G10
WM-115 Vemurafenib I-BET151 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -27.37 NGFR rabbit IgG(H+L) / donkey NGFR 768.94
WM115-P3
G11
WM-115 Vemurafenib (+)-JQ1 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 155.02 NGFR rabbit IgG(H+L) / donkey NGFR 1073.92
WM115-P3
G2
WM-115 Vemurafenib (+)-JQ1 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 133.91 NGFR rabbit IgG(H+L) / donkey NGFR 998.17
WM115-P3
G3
WM-115 Vemurafenib (+)-JQ1 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 72.41 NGFR rabbit IgG(H+L) / donkey NGFR 1028.41
WM115-P3
G4
WM-115 Vemurafenib (+)-JQ1 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 100.21 NGFR rabbit IgG(H+L) / donkey NGFR 1075.48
WM115-P3
G5
WM-115 Vemurafenib (+)-JQ1 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 90.47 NGFR rabbit IgG(H+L) / donkey NGFR 1018.11
WM115-P3
G6
WM-115 Vemurafenib I-BET151 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 174.82 NGFR rabbit IgG(H+L) / donkey NGFR 979.89
WM115-P3
G7
WM-115 Vemurafenib I-BET151 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 192.89 NGFR rabbit IgG(H+L) / donkey NGFR 913.19
WM115-P3
G8
WM-115 Vemurafenib I-BET151 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 65.90 NGFR rabbit IgG(H+L) / donkey NGFR 968.88
WM115-P3
G9
WM-115 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 21.21 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 406.64
WM115-P4
C10
WM-115 Vemurafenib Trametinib 3.160 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -30.47 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 272.96
WM115-P4
C11
WM-115 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 323.68 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1577.51
WM115-P4
C2
WM-115 Vemurafenib 0.100
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 232.70 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1064.13
WM115-P4
C3
WM-115 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.71 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 572.25
WM115-P4
C4
WM-115 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 52.34 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 378.59
WM115-P4
C5
WM-115 Vemurafenib 3.160
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 21.07 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 291.78
WM115-P4
C6
WM-115 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 26.63 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 460.08
WM115-P4
C7
WM-115 Vemurafenib Trametinib 0.100 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 57.99 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 439.48
WM115-P4
C8