Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 4 of 4) - Dataset (ID:20277)

 Full screen view
Filter results: >>>>
Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein 1 Detected (647) Protein 2 Detected (647) Protein 3 Detected (647) Protein 4 Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 154.17 NGFR rabbit IgG(H+L) / donkey NGFR 653.89
A375-P3
B5
A-375 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1317.78 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 172.36
A375-P2
B2
A-375 Vemurafenib 3.160
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.56 NGFR rabbit IgG(H+L) / donkey NGFR 435.84
A375-P3
B6
WM-115 Vemurafenib I-BET151 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 124.20 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 743.84
WM115-P1
F7
A-375 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 100.84 NGFR rabbit IgG(H+L) / donkey NGFR 788.23
A375-P3
B7
A-375 Vemurafenib 0.100
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 712.85 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 402.29
A375-P2
B3
A-375 Vemurafenib Trametinib 0.100 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 111.16 NGFR rabbit IgG(H+L) / donkey NGFR 712.13
A375-P3
B8
COLO 858 Vemurafenib I-BET151 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 240.34 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 31.33
Colo858-P1
F11
A-375 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 63.33 NGFR rabbit IgG(H+L) / donkey NGFR 731.89
A375-P3
B9
COLO 858 Vemurafenib I-BET 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 293.26 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 48.43
Colo858-P1
D10
COLO 858 Vemurafenib I-BET 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 269.76 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 38.60
Colo858-P1
D11
COLO 858 Vemurafenib Belinostat 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 373.46 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 656.78
Colo858-P1
D2
COLO 858 Vemurafenib Belinostat 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 356.63 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 134.14
Colo858-P1
D3
COLO 858 Vemurafenib Belinostat 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 370.58 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 66.51
Colo858-P1
D4
COLO 858 Vemurafenib Belinostat 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 233.75 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 50.00
Colo858-P1
D5
COLO 858 Vemurafenib Belinostat 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 327.09 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 48.82
Colo858-P1
D6
COLO 858 Vemurafenib I-BET 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 885.68 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 768.92
Colo858-P1
D7
COLO 858 Vemurafenib I-BET 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 228.73 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 78.63
Colo858-P1
D8
COLO 858 Vemurafenib I-BET 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 244.15 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 45.96
Colo858-P1
D9
COLO 858 Vemurafenib I-BET151 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 600.64 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 749.91
Colo858-P1
F7
A-375 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.55 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 902.23
A375-P2
B7
COLO 858 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1420.93 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 94.42
Colo858-P2
B2
A-375 Vemurafenib 0.100
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 704.46 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 118.84
A375-P1
B3
COLO 858 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 414.29 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 471.30
Colo858-P2
B4
WM-115 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 163.26 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 227.60
WM115-P2
B4