Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 4 of 4) - Dataset (ID:20277)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein 1 Detected (647) Protein 2 Detected (647) Protein 3 Detected (647) Protein 4 Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 65.07 NGFR rabbit IgG(H+L) / donkey NGFR 2077.24
WM115-P3
C9
WM-115 Vemurafenib I-BET 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 34.81 NGFR rabbit IgG(H+L) / donkey NGFR 1187.71
WM115-P3
D10
WM-115 Vemurafenib I-BET 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 5.32 NGFR rabbit IgG(H+L) / donkey NGFR 843.16
WM115-P3
D11
WM-115 Vemurafenib Belinostat 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 160.34 NGFR rabbit IgG(H+L) / donkey NGFR 908.82
WM115-P3
D2
WM-115 Vemurafenib Belinostat 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 132.65 NGFR rabbit IgG(H+L) / donkey NGFR 870.13
WM115-P3
D3
WM-115 Vemurafenib Belinostat 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 92.49 NGFR rabbit IgG(H+L) / donkey NGFR 771.16
WM115-P3
D4
WM-115 Vemurafenib Belinostat 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 15.84 NGFR rabbit IgG(H+L) / donkey NGFR 703.59
WM115-P3
D5
WM-115 Vemurafenib Belinostat 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -37.79 NGFR rabbit IgG(H+L) / donkey NGFR 665.16
WM115-P3
D6
WM-115 Vemurafenib I-BET 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 93.10 NGFR rabbit IgG(H+L) / donkey NGFR 916.16
WM115-P3
D7
WM-115 Vemurafenib I-BET 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 111.23 NGFR rabbit IgG(H+L) / donkey NGFR 968.59
WM115-P3
D8
WM-115 Vemurafenib I-BET 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 30.80 NGFR rabbit IgG(H+L) / donkey NGFR 1167.95
WM115-P3
E10
WM-115 Vemurafenib I-BET 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -12.46 NGFR rabbit IgG(H+L) / donkey NGFR 896.89
WM115-P3
E11
WM-115 Vemurafenib Belinostat 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 125.00 NGFR rabbit IgG(H+L) / donkey NGFR 854.12
WM115-P3
E2
WM-115 Vemurafenib Belinostat 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 112.69 NGFR rabbit IgG(H+L) / donkey NGFR 869.14
WM115-P3
E3
WM-115 Vemurafenib Belinostat 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 72.12 NGFR rabbit IgG(H+L) / donkey NGFR 770.43
WM115-P3
E4
WM-115 Vemurafenib Belinostat 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 32.44 NGFR rabbit IgG(H+L) / donkey NGFR 724.88
WM115-P3
E5
WM-115 Vemurafenib Belinostat 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 11.76 NGFR rabbit IgG(H+L) / donkey NGFR 666.72
WM115-P3
E6
WM-115 Vemurafenib I-BET 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 111.82 NGFR rabbit IgG(H+L) / donkey NGFR 866.72
WM115-P3
E7
WM-115 Vemurafenib I-BET 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 107.98 NGFR rabbit IgG(H+L) / donkey NGFR 918.12
WM115-P3
E8
WM-115 Vemurafenib I-BET 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 51.30 NGFR rabbit IgG(H+L) / donkey NGFR 1033.29
WM115-P3
E9
WM-115 Vemurafenib I-BET151 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1.36 NGFR rabbit IgG(H+L) / donkey NGFR 979.07
WM115-P3
F10
WM-115 Vemurafenib I-BET151 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -20.88 NGFR rabbit IgG(H+L) / donkey NGFR 797.93
WM115-P3
F11
WM-115 Vemurafenib (+)-JQ1 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 200.27 NGFR rabbit IgG(H+L) / donkey NGFR 1110.47
WM115-P3
F2
WM-115 Vemurafenib (+)-JQ1 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 144.54 NGFR rabbit IgG(H+L) / donkey NGFR 976.08
WM115-P3
F3
WM-115 Vemurafenib (+)-JQ1 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 89.66 NGFR rabbit IgG(H+L) / donkey NGFR 1051.66
WM115-P3
F4