Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 4 of 4) - Dataset (ID:20277)

 Full screen view
Filter results: >>>>
Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein 1 Detected (647) Protein 2 Detected (647) Protein 3 Detected (647) Protein 4 Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Trametinib 0.100 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 71.77 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 860.61
A375-P2
C8
WM-115 Vemurafenib Trametinib 0.100 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 60.13 NGFR rabbit IgG(H+L) / donkey NGFR 2045.24
WM115-P3
C8
A-375 Vemurafenib Trametinib 0.100 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 92.75 NGFR rabbit IgG(H+L) / donkey NGFR 779.46
A375-P3
C8
WM-115 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 56.08 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 441.56
WM115-P4
C9
WM-115 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 65.07 NGFR rabbit IgG(H+L) / donkey NGFR 2077.24
WM115-P3
C9
COLO 858 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 242.08 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 749.04
Colo858-P2
C9
WM-115 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 34.06 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) -1.91
WM115-P1
C9
COLO 858 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 194.94 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 171.85
Colo858-P4
C9
WM-115 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 41.21 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 204.84
WM115-P2
C9
A-375 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -10.37 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 445.94
A375-P4
C9
A-375 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 137.06 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 18.81
A375-P1
C9
COLO 858 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 251.36 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 36.42
Colo858-P1
C9
A-375 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 58.85 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 831.79
A375-P2
C9
COLO 858 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 210.18 NGFR rabbit IgG(H+L) / donkey NGFR 376.06
Colo858-P3
C9
A-375 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 81.62 NGFR rabbit IgG(H+L) / donkey NGFR 745.88
A375-P3
C9
WM-115 Vemurafenib I-BET 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 34.81 NGFR rabbit IgG(H+L) / donkey NGFR 1187.71
WM115-P3
D10
COLO 858 Vemurafenib I-BET 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 104.48 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 299.20
Colo858-P4
D10
WM-115 Vemurafenib I-BET 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 38.15 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 20.58
WM115-P1
D10
COLO 858 Vemurafenib I-BET 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 210.47 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 287.24
Colo858-P2
D10
A-375 Vemurafenib I-BET 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -19.84 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 260.20
A375-P4
D10
COLO 858 Vemurafenib I-BET 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 158.50 NGFR rabbit IgG(H+L) / donkey NGFR 28.79
Colo858-P3
D10
WM-115 Vemurafenib I-BET 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 39.53 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 219.68
WM115-P2
D10
A-375 Vemurafenib I-BET 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 133.62 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 34.79
A375-P1
D10
COLO 858 Vemurafenib I-BET 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 293.26 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 48.43
Colo858-P1
D10
A-375 Vemurafenib I-BET 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 56.25 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 364.21
A375-P2
D10