Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 4 of 4) - Dataset (ID:20277)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein 1 Detected (647) Protein 2 Detected (647) Protein 3 Detected (647) Protein 4 Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib I-BET 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 961.05 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 3564.85
Colo858-P4
E7
COLO 858 Vemurafenib I-BET 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 990.02 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 3394.66
Colo858-P4
D7
COLO 858 Vemurafenib I-BET 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1038.62 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 88.14
Colo858-P2
D7
COLO 858 Vemurafenib I-BET 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1061.26 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 85.04
Colo858-P2
E7
A-375 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1197.20 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 2466.67
A375-P4
C2
A-375 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1220.77 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 2378.45
A375-P4
B2
COLO 858 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1226.36 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 797.35
Colo858-P1
B2
A-375 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1265.78 NGFR rabbit IgG(H+L) / donkey NGFR 358.34
A375-P3
C2
A-375 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1315.15 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 172.98
A375-P2
C2
A-375 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1317.78 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 172.36
A375-P2
B2
A-375 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1328.75 NGFR rabbit IgG(H+L) / donkey NGFR 386.89
A375-P3
B2
A-375 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1334.95 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 684.50
A375-P1
B2
COLO 858 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1349.54 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 2836.10
Colo858-P4
C2
COLO 858 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1380.77 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 2910.42
Colo858-P4
B2
A-375 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1389.99 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 682.68
A375-P1
C2
COLO 858 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1397.59 NGFR rabbit IgG(H+L) / donkey NGFR 52.78
Colo858-P3
C2
COLO 858 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1403.63 NGFR rabbit IgG(H+L) / donkey NGFR 64.31
Colo858-P3
B2
COLO 858 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1420.93 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 94.42
Colo858-P2
B2
COLO 858 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1422.88 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 802.25
Colo858-P1
C2
COLO 858 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1478.74 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 103.01
Colo858-P2
C2