Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 4 of 4) - Dataset (ID:20277)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein 1 Detected (647) Protein 2 Detected (647) Protein 3 Detected (647) Protein 4 Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib I-BET151 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 98.71 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 39.14
A375-P1
G10
WM-115 Vemurafenib I-BET151 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 99.09 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 199.40
WM115-P2
F8
WM-115 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 99.91 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 212.64
WM115-P2
B7
WM-115 Vemurafenib (+)-JQ1 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 100.21 NGFR rabbit IgG(H+L) / donkey NGFR 1075.48
WM115-P3
G5
A-375 Vemurafenib 3.160
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 100.24 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 489.26
A375-P2
B6
WM-115 Vemurafenib (+)-JQ1 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 100.26 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 221.21
WM115-P2
F4
WM-115 Vemurafenib I-BET 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 100.50 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 785.95
WM115-P1
D7
A-375 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 100.84 NGFR rabbit IgG(H+L) / donkey NGFR 788.23
A375-P3
B7
A-375 Vemurafenib (+)-JQ1 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 101.16 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 265.02
A375-P2
F5
A-375 Vemurafenib Trametinib 0.100 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 101.47 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 860.33
A375-P2
B8
WM-115 Vemurafenib I-BET 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 102.25 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 779.55
WM115-P1
E7
WM-115 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 102.48 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 337.63
WM115-P4
B5
COLO 858 Vemurafenib Trametinib 3.160 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 102.66 NGFR rabbit IgG(H+L) / donkey NGFR 69.04
Colo858-P3
B11
WM-115 Vemurafenib (+)-JQ1 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 103.17 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 575.54
WM115-P4
G5
COLO 858 Vemurafenib I-BET 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 104.48 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 299.20
Colo858-P4
D10
WM-115 Vemurafenib 3.160
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 104.85 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 290.79
WM115-P4
B6
A-375 Vemurafenib Belinostat 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 105.57 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 50.55
A375-P1
E5
WM-115 Vemurafenib (+)-JQ1 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 105.61 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 423.11
WM115-P4
G6
WM-115 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 105.75 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 465.18
WM115-P4
B7
WM-115 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 107.90 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 207.30
WM115-P2
B5
WM-115 Vemurafenib I-BET 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 107.98 NGFR rabbit IgG(H+L) / donkey NGFR 918.12
WM115-P3
E8
WM-115 Vemurafenib I-BET 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 108.47 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 208.22
WM115-P2
D8
A-375 Vemurafenib Belinostat 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 108.94 NGFR rabbit IgG(H+L) / donkey NGFR 35.10
A375-P3
E4
WM-115 Vemurafenib I-BET151 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 109.16 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 64.88
WM115-P2
F7
A-375 Vemurafenib 3.160
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 110.57 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 45.79
A375-P1
C6