Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 4 of 4) - Dataset (ID:20277)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein 1 Detected (647) Protein 2 Detected (647) Protein 3 Detected (647) Protein 4 Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib I-BET151 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 204.04 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 192.98
Colo858-P2
F10
COLO 858 Vemurafenib (+)-JQ1 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 331.50 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 300.70
Colo858-P2
F5
COLO 858 Vemurafenib I-BET151 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 218.04 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 228.57
Colo858-P2
G10
COLO 858 Vemurafenib (+)-JQ1 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 347.98 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 214.70
Colo858-P2
G5
COLO 858 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 173.30 NGFR rabbit IgG(H+L) / donkey NGFR 320.04
Colo858-P3
B10
COLO 858 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 300.75 NGFR rabbit IgG(H+L) / donkey NGFR 154.41
Colo858-P3
B5
COLO 858 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 188.02 NGFR rabbit IgG(H+L) / donkey NGFR 382.19
Colo858-P3
C10
COLO 858 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 224.45 NGFR rabbit IgG(H+L) / donkey NGFR 168.67
Colo858-P3
C5
COLO 858 Vemurafenib I-BET 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 158.50 NGFR rabbit IgG(H+L) / donkey NGFR 28.79
Colo858-P3
D10
COLO 858 Vemurafenib Belinostat 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 220.94 NGFR rabbit IgG(H+L) / donkey NGFR 94.00
Colo858-P3
D5
COLO 858 Vemurafenib I-BET 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 170.61 NGFR rabbit IgG(H+L) / donkey NGFR 25.12
Colo858-P3
E10
COLO 858 Vemurafenib Belinostat 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 176.07 NGFR rabbit IgG(H+L) / donkey NGFR 75.51
Colo858-P3
E5
COLO 858 Vemurafenib I-BET151 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 193.79 NGFR rabbit IgG(H+L) / donkey NGFR 26.55
Colo858-P3
F10
COLO 858 Vemurafenib (+)-JQ1 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 241.50 NGFR rabbit IgG(H+L) / donkey NGFR 44.91
Colo858-P3
F5
COLO 858 Vemurafenib I-BET151 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 190.47 NGFR rabbit IgG(H+L) / donkey NGFR 24.44
Colo858-P3
G10
COLO 858 Vemurafenib (+)-JQ1 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 311.55 NGFR rabbit IgG(H+L) / donkey NGFR 51.37
Colo858-P3
G5
COLO 858 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 150.23 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 188.77
Colo858-P4
B10
COLO 858 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 290.52 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 269.09
Colo858-P4
B5
COLO 858 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 175.95 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 148.80
Colo858-P4
C10
COLO 858 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 279.71 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 268.34
Colo858-P4
C5
COLO 858 Vemurafenib I-BET 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 104.48 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 299.20
Colo858-P4
D10
COLO 858 Vemurafenib Belinostat 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 191.61 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 856.49
Colo858-P4
D5
A-375 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 87.44 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 16.69
A375-P1
B10
COLO 858 Vemurafenib Belinostat 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 281.47 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 894.01
Colo858-P4
E5
COLO 858 Vemurafenib I-BET151 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 194.47 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 371.62
Colo858-P4
F10