Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 4 of 4) - Dataset (ID:20277)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein 1 Detected (647) Protein 2 Detected (647) Protein 3 Detected (647) Protein 4 Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib 0.100
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 704.46 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 118.84
A375-P1
B3
WM-115 Vemurafenib Trametinib 0.100 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 76.98 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 2.33
WM115-P1
B8
A-375 Vemurafenib Trametinib 0.100 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 92.75 NGFR rabbit IgG(H+L) / donkey NGFR 779.46
A375-P3
C8
COLO 858 Vemurafenib I-BET 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 177.36 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1401.44
Colo858-P4
D8
WM-115 Vemurafenib 0.100
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 250.32 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 171.29
WM115-P1
B3
COLO 858 Vemurafenib I-BET151 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 178.82 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1305.82
Colo858-P4
G8
A-375 Vemurafenib Belinostat 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 250.02 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 150.27
A375-P1
E3
COLO 858 Vemurafenib Belinostat 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 382.99 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 167.43
Colo858-P1
E3
COLO 858 Vemurafenib (+)-JQ1 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 389.55 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 109.83
Colo858-P1
F3
COLO 858 Vemurafenib I-BET151 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 283.48 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 217.07
Colo858-P2
G8
COLO 858 Vemurafenib (+)-JQ1 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 247.74 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1599.29
Colo858-P4
F3
COLO 858 Vemurafenib I-BET 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 228.73 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 78.63
Colo858-P1
D8
A-375 Vemurafenib 0.100
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 488.72 NGFR rabbit IgG(H+L) / donkey NGFR 484.00
A375-P3
C3
COLO 858 Vemurafenib I-BET151 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 192.86 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1464.16
Colo858-P4
F8
COLO 858 Vemurafenib I-BET 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 237.48 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 71.38
Colo858-P1
E8
COLO 858 Vemurafenib I-BET151 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 257.94 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 242.40
Colo858-P2
F8
COLO 858 Vemurafenib Belinostat 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 356.63 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 134.14
Colo858-P1
D3
COLO 858 Vemurafenib (+)-JQ1 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 321.42 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 238.89
Colo858-P2
F3
COLO 858 Vemurafenib 0.100
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 879.72 NGFR rabbit IgG(H+L) / donkey NGFR 71.30
Colo858-P3
C3
COLO 858 Vemurafenib (+)-JQ1 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 394.95 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 102.65
Colo858-P1
G3
A-375 Vemurafenib 0.100
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 712.85 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 402.29
A375-P2
B3
COLO 858 Vemurafenib (+)-JQ1 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 330.58 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 227.73
Colo858-P2
G3
COLO 858 Vemurafenib (+)-JQ1 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 284.54 NGFR rabbit IgG(H+L) / donkey NGFR 42.93
Colo858-P3
F3
COLO 858 Vemurafenib Trametinib 0.100 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 270.32 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 31.77
Colo858-P1
C8
A-375 Vemurafenib Trametinib 0.100 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 111.16 NGFR rabbit IgG(H+L) / donkey NGFR 712.13
A375-P3
B8