Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 4 of 4) - Dataset (ID:20277)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein 1 Detected (647) Protein 2 Detected (647) Protein 3 Detected (647) Protein 4 Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 154.17 NGFR rabbit IgG(H+L) / donkey NGFR 653.89
A375-P3
B5
WM-115 Vemurafenib Belinostat 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 92.49 NGFR rabbit IgG(H+L) / donkey NGFR 771.16
WM115-P3
D4
A-375 Vemurafenib 3.160
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.56 NGFR rabbit IgG(H+L) / donkey NGFR 435.84
A375-P3
B6
WM-115 Vemurafenib Belinostat 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -37.79 NGFR rabbit IgG(H+L) / donkey NGFR 665.16
WM115-P3
D6
A-375 Vemurafenib I-BET151 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 745.91 NGFR rabbit IgG(H+L) / donkey NGFR 245.85
A375-P3
G7
A-375 Vemurafenib (+)-JQ1 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 86.41 NGFR rabbit IgG(H+L) / donkey NGFR 79.59
A375-P3
G6
A-375 Vemurafenib (+)-JQ1 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 98.60 NGFR rabbit IgG(H+L) / donkey NGFR 146.35
A375-P3
G5
COLO 858 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 391.25 NGFR rabbit IgG(H+L) / donkey NGFR 105.88
Colo858-P3
B4
A-375 Vemurafenib (+)-JQ1 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 119.57 NGFR rabbit IgG(H+L) / donkey NGFR 119.71
A375-P3
G4
A-375 Vemurafenib (+)-JQ1 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 193.38 NGFR rabbit IgG(H+L) / donkey NGFR 97.06
A375-P3
G3
A-375 Vemurafenib (+)-JQ1 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 374.84 NGFR rabbit IgG(H+L) / donkey NGFR 191.92
A375-P3
G2
A-375 Vemurafenib I-BET151 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -8.03 NGFR rabbit IgG(H+L) / donkey NGFR 84.94
A375-P3
G11
A-375 Vemurafenib I-BET151 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 40.15 NGFR rabbit IgG(H+L) / donkey NGFR 154.03
A375-P3
G10
A-375 Vemurafenib I-BET151 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 180.04 NGFR rabbit IgG(H+L) / donkey NGFR 144.88
A375-P3
F9
A-375 Vemurafenib I-BET151 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 278.95 NGFR rabbit IgG(H+L) / donkey NGFR 93.45
A375-P3
F8
A-375 Vemurafenib I-BET151 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 718.83 NGFR rabbit IgG(H+L) / donkey NGFR 240.96
A375-P3
F7
A-375 Vemurafenib (+)-JQ1 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 46.97 NGFR rabbit IgG(H+L) / donkey NGFR 88.97
A375-P3
F6
A-375 Vemurafenib (+)-JQ1 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 79.95 NGFR rabbit IgG(H+L) / donkey NGFR 158.37
A375-P3
F5
A-375 Vemurafenib (+)-JQ1 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 141.85 NGFR rabbit IgG(H+L) / donkey NGFR 125.72
A375-P3
F4
A-375 Vemurafenib (+)-JQ1 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 206.48 NGFR rabbit IgG(H+L) / donkey NGFR 104.55
A375-P3
F3
A-375 Vemurafenib (+)-JQ1 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 371.71 NGFR rabbit IgG(H+L) / donkey NGFR 192.81
A375-P3
F2
A-375 Vemurafenib I-BET151 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3.01 NGFR rabbit IgG(H+L) / donkey NGFR 76.51
A375-P3
F11
A-375 Vemurafenib I-BET151 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 91.25 NGFR rabbit IgG(H+L) / donkey NGFR 167.27
A375-P3
F10
A-375 Vemurafenib I-BET 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 155.78 NGFR rabbit IgG(H+L) / donkey NGFR 266.72
A375-P3
E9
COLO 858 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 300.75 NGFR rabbit IgG(H+L) / donkey NGFR 154.41
Colo858-P3
B5