Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 4 of 4) - Dataset (ID:20277)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein 1 Detected (647) Protein 2 Detected (647) Protein 3 Detected (647) Protein 4 Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib I-BET 0.316 1.000
uM
48
hr
2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 218.03 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 295.01
Colo858-P2
E9
COLO 858 Vemurafenib I-BET151 1.000 1.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 204.04 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 192.98
Colo858-P2
F10
COLO 858 Vemurafenib I-BET151 3.160 1.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 155.51 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 225.16
Colo858-P2
F11
COLO 858 Vemurafenib (+)-JQ1 0.000 1.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 458.84 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 123.06
Colo858-P2
F2
COLO 858 Vemurafenib (+)-JQ1 0.100 1.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 321.42 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 238.89
Colo858-P2
F3
COLO 858 Vemurafenib (+)-JQ1 0.316 1.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 269.44 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 218.26
Colo858-P2
F4
COLO 858 Vemurafenib (+)-JQ1 1.000 1.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 331.50 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 300.70
Colo858-P2
F5
COLO 858 Vemurafenib (+)-JQ1 3.160 1.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 275.55 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 331.66
Colo858-P2
F6
COLO 858 Vemurafenib I-BET151 0.000 1.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 609.36 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 102.60
Colo858-P2
F7
COLO 858 Vemurafenib I-BET151 0.100 1.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 257.94 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 242.40
Colo858-P2
F8
COLO 858 Vemurafenib I-BET151 0.316 1.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 239.36 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 219.34
Colo858-P2
F9
COLO 858 Vemurafenib I-BET151 1.000 1.000
uM
48
hr
2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 218.04 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 228.57
Colo858-P2
G10
COLO 858 Vemurafenib I-BET151 3.160 1.000
uM
48
hr
2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 152.08 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 211.05
Colo858-P2
G11
COLO 858 Vemurafenib (+)-JQ1 0.000 1.000
uM
48
hr
2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 451.17 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 96.64
Colo858-P2
G2
COLO 858 Vemurafenib (+)-JQ1 0.100 1.000
uM
48
hr
2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 330.58 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 227.73
Colo858-P2
G3
COLO 858 Vemurafenib (+)-JQ1 0.316 1.000
uM
48
hr
2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 290.48 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 207.76
Colo858-P2
G4
COLO 858 Vemurafenib (+)-JQ1 1.000 1.000
uM
48
hr
2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 347.98 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 214.70
Colo858-P2
G5
COLO 858 Vemurafenib (+)-JQ1 3.160 1.000
uM
48
hr
2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 314.66 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 290.86
Colo858-P2
G6
COLO 858 Vemurafenib I-BET151 0.000 1.000
uM
48
hr
2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 690.88 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 98.91
Colo858-P2
G7
COLO 858 Vemurafenib I-BET151 0.100 1.000
uM
48
hr
2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 283.48 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 217.07
Colo858-P2
G8