Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 4 of 4) - Dataset (ID:20277)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein 1 Detected (647) Protein 2 Detected (647) Protein 3 Detected (647) Protein 4 Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib I-BET151 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 66.00 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 320.00
A375-P2
F10
A-375 Vemurafenib I-BET151 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1.94 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 291.04
A375-P2
F11
A-375 Vemurafenib (+)-JQ1 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 448.98 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 69.95
A375-P2
F2
A-375 Vemurafenib (+)-JQ1 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 349.87 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 173.78
A375-P2
F3
A-375 Vemurafenib (+)-JQ1 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 159.35 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 196.41
A375-P2
F4
A-375 Vemurafenib (+)-JQ1 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 101.16 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 265.02
A375-P2
F5
A-375 Vemurafenib (+)-JQ1 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 62.67 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 293.21
A375-P2
F6
A-375 Vemurafenib I-BET151 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 837.08 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 71.66
A375-P2
F7
A-375 Vemurafenib I-BET151 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 426.59 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 226.72
A375-P2
F8
A-375 Vemurafenib I-BET151 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 226.73 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 298.06
A375-P2
F9
A-375 Vemurafenib I-BET151 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 89.41 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 298.73
A375-P2
G10
A-375 Vemurafenib I-BET151 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -3.33 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 289.86
A375-P2
G11
A-375 Vemurafenib (+)-JQ1 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 409.38 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 61.89
A375-P2
G2
A-375 Vemurafenib (+)-JQ1 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 261.49 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 125.02
A375-P2
G3
A-375 Vemurafenib (+)-JQ1 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 144.33 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 189.72
A375-P2
G4
A-375 Vemurafenib (+)-JQ1 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 145.71 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 244.02
A375-P2
G5
A-375 Vemurafenib (+)-JQ1 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 98.71 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 288.00
A375-P2
G6
A-375 Vemurafenib I-BET151 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 896.67 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 76.70
A375-P2
G7
A-375 Vemurafenib I-BET151 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 529.36 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 231.16
A375-P2
G8
A-375 Vemurafenib I-BET151 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 216.96 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 286.88
A375-P2
G9
A-375 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 21.32 NGFR rabbit IgG(H+L) / donkey NGFR 582.87
A375-P3
B10
A-375 Vemurafenib Trametinib 3.160 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -50.96 NGFR rabbit IgG(H+L) / donkey NGFR 377.54
A375-P3
B11
A-375 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1328.75 NGFR rabbit IgG(H+L) / donkey NGFR 386.89
A375-P3
B2
A-375 Vemurafenib 0.100
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 586.19 NGFR rabbit IgG(H+L) / donkey NGFR 493.73
A375-P3
B3
A-375 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 325.95 NGFR rabbit IgG(H+L) / donkey NGFR 687.57
A375-P3
B4