Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 4 of 4) - Dataset (ID:20277)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein 1 Detected (647) Protein 2 Detected (647) Protein 3 Detected (647) Protein 4 Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib I-BET151 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 193.79 NGFR rabbit IgG(H+L) / donkey NGFR 26.55
Colo858-P3
F10
COLO 858 Vemurafenib I-BET151 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 162.48 NGFR rabbit IgG(H+L) / donkey NGFR 22.52
Colo858-P3
F11
COLO 858 Vemurafenib (+)-JQ1 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 438.07 NGFR rabbit IgG(H+L) / donkey NGFR 58.73
Colo858-P3
F2
COLO 858 Vemurafenib (+)-JQ1 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 284.54 NGFR rabbit IgG(H+L) / donkey NGFR 42.93
Colo858-P3
F3
COLO 858 Vemurafenib (+)-JQ1 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 234.36 NGFR rabbit IgG(H+L) / donkey NGFR 43.02
Colo858-P3
F4
COLO 858 Vemurafenib (+)-JQ1 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 241.50 NGFR rabbit IgG(H+L) / donkey NGFR 44.91
Colo858-P3
F5
COLO 858 Vemurafenib (+)-JQ1 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 237.87 NGFR rabbit IgG(H+L) / donkey NGFR 33.42
Colo858-P3
F6
COLO 858 Vemurafenib I-BET151 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 624.30 NGFR rabbit IgG(H+L) / donkey NGFR 55.39
Colo858-P3
F7
COLO 858 Vemurafenib I-BET151 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 207.25 NGFR rabbit IgG(H+L) / donkey NGFR 32.37
Colo858-P3
F8
COLO 858 Vemurafenib I-BET151 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 201.44 NGFR rabbit IgG(H+L) / donkey NGFR 29.70
Colo858-P3
F9
COLO 858 Vemurafenib I-BET151 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 190.47 NGFR rabbit IgG(H+L) / donkey NGFR 24.44
Colo858-P3
G10
COLO 858 Vemurafenib I-BET151 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 147.81 NGFR rabbit IgG(H+L) / donkey NGFR 23.36
Colo858-P3
G11
COLO 858 Vemurafenib (+)-JQ1 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 387.06 NGFR rabbit IgG(H+L) / donkey NGFR 65.52
Colo858-P3
G2
COLO 858 Vemurafenib (+)-JQ1 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 275.03 NGFR rabbit IgG(H+L) / donkey NGFR 40.26
Colo858-P3
G3
COLO 858 Vemurafenib (+)-JQ1 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 235.91 NGFR rabbit IgG(H+L) / donkey NGFR 38.62
Colo858-P3
G4
COLO 858 Vemurafenib (+)-JQ1 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 311.55 NGFR rabbit IgG(H+L) / donkey NGFR 51.37
Colo858-P3
G5
COLO 858 Vemurafenib (+)-JQ1 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 282.32 NGFR rabbit IgG(H+L) / donkey NGFR 42.28
Colo858-P3
G6
COLO 858 Vemurafenib I-BET151 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 617.29 NGFR rabbit IgG(H+L) / donkey NGFR 58.12
Colo858-P3
G7
COLO 858 Vemurafenib I-BET151 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 221.97 NGFR rabbit IgG(H+L) / donkey NGFR 31.69
Colo858-P3
G8
COLO 858 Vemurafenib I-BET151 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 194.52 NGFR rabbit IgG(H+L) / donkey NGFR 31.49
Colo858-P3
G9
COLO 858 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 150.23 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 188.77
Colo858-P4
B10
COLO 858 Vemurafenib Trametinib 3.160 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2.87 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 25.49
Colo858-P4
B11
COLO 858 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1380.77 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 2910.42
Colo858-P4
B2
COLO 858 Vemurafenib 0.100
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 752.34 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 945.89
Colo858-P4
B3
COLO 858 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 461.80 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 499.07
Colo858-P4
B4