Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 4 of 4) - Dataset (ID:20277)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein 1 Detected (647) Protein 2 Detected (647) Protein 3 Detected (647) Protein 4 Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Belinostat 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 307.15 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 98.50
Colo858-P2
D5
COLO 858 Vemurafenib Belinostat 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 273.49 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 85.90
Colo858-P2
D6
COLO 858 Vemurafenib I-BET 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1038.62 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 88.14
Colo858-P2
D7
COLO 858 Vemurafenib I-BET 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 229.31 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 297.57
Colo858-P2
D8
COLO 858 Vemurafenib I-BET 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 228.48 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 301.56
Colo858-P2
D9
COLO 858 Vemurafenib I-BET 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 202.32 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 264.52
Colo858-P2
E10
COLO 858 Vemurafenib I-BET 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 205.04 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 290.14
Colo858-P2
E11
COLO 858 Vemurafenib Belinostat 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 388.19 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 183.54
Colo858-P2
E2
COLO 858 Vemurafenib Belinostat 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 353.34 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 225.20
Colo858-P2
E3
COLO 858 Vemurafenib Belinostat 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 319.46 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 172.15
Colo858-P2
E4
COLO 858 Vemurafenib Belinostat 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 215.65 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 117.26
Colo858-P2
E5
COLO 858 Vemurafenib Belinostat 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 186.26 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 90.70
Colo858-P2
E6
COLO 858 Vemurafenib I-BET 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1061.26 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 85.04
Colo858-P2
E7
COLO 858 Vemurafenib I-BET 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 245.38 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 313.24
Colo858-P2
E8
COLO 858 Vemurafenib I-BET 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 218.03 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 295.01
Colo858-P2
E9
COLO 858 Vemurafenib I-BET151 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 204.04 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 192.98
Colo858-P2
F10
COLO 858 Vemurafenib I-BET151 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 155.51 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 225.16
Colo858-P2
F11
COLO 858 Vemurafenib (+)-JQ1 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 458.84 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 123.06
Colo858-P2
F2
COLO 858 Vemurafenib (+)-JQ1 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 321.42 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 238.89
Colo858-P2
F3
COLO 858 Vemurafenib (+)-JQ1 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 269.44 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 218.26
Colo858-P2
F4
COLO 858 Vemurafenib (+)-JQ1 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 331.50 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 300.70
Colo858-P2
F5
COLO 858 Vemurafenib (+)-JQ1 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 275.55 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 331.66
Colo858-P2
F6
COLO 858 Vemurafenib I-BET151 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 609.36 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 102.60
Colo858-P2
F7
COLO 858 Vemurafenib I-BET151 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 257.94 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 242.40
Colo858-P2
F8
COLO 858 Vemurafenib I-BET151 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 239.36 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 219.34
Colo858-P2
F9