Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib GSK-J2 1.0000 0.5333
uM
 48
hr
 1 1420 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 133.29 NGFR rabbit IgG(H+L) / donkey NGFR 997.01
WM115-B1-rep1
C6
WM-115 Vemurafenib (+)-JQ1 1.0000 0.5333
uM
 48
hr
 1 878 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 133.24 NGFR rabbit IgG(H+L) / donkey NGFR 474.49
WM115-B1-rep1
C7
WM-115 Vemurafenib UNC1215 1.0000 0.5333
uM
 48
hr
 1 1417 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.34 NGFR rabbit IgG(H+L) / donkey NGFR 1116.16
WM115-B1-rep1
C8
WM-115 Vemurafenib Vorinostat 1.0000 0.5333
uM
 48
hr
 1 1611 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.96 NGFR rabbit IgG(H+L) / donkey NGFR 915.06
WM115-B1-rep1
D2
WM-115 Vemurafenib JW55 1.0000 0.5333
uM
 48
hr
 1 1522 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 143.33 NGFR rabbit IgG(H+L) / donkey NGFR 1091.59
WM115-B1-rep1
D3
WM-115 Vemurafenib Entinostat 1.0000 0.5333
uM
 48
hr
 1 1649 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 125.31 NGFR rabbit IgG(H+L) / donkey NGFR 1011.83
WM115-B1-rep1
D4
WM-115 Vemurafenib AGK2 1.0000 0.5333
uM
 48
hr
 1 915 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 145.02 NGFR rabbit IgG(H+L) / donkey NGFR 1130.32
WM115-B1-rep1
D5
WM-115 Vemurafenib GSK-J4 1.0000 0.5333
uM
 48
hr
 1 1681 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 133.56 NGFR rabbit IgG(H+L) / donkey NGFR 1168.38
WM115-B1-rep1
D6
WM-115 Vemurafenib (-)-JQ1 1.0000 0.5333
uM
 48
hr
 1 1613 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.43 NGFR rabbit IgG(H+L) / donkey NGFR 1200.88
WM115-B1-rep1
D7
WM-115 Vemurafenib IOX2 1.0000 0.5333
uM
 48
hr
 1 1449 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 146.49 NGFR rabbit IgG(H+L) / donkey NGFR 1057.33
WM115-B1-rep1
D8
WM-115 Vemurafenib Azacitidine 1.0000 0.5333
uM
 48
hr
 1 1281 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 161.75 NGFR rabbit IgG(H+L) / donkey NGFR 938.35
WM115-B1-rep1
E2
WM-115 Vemurafenib C646 1.0000 0.5333
uM
 48
hr
 1 1210 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 130.90 NGFR rabbit IgG(H+L) / donkey NGFR 932.39
WM115-B1-rep1
E3
WM-115 Vemurafenib Mocetinostat 1.0000 0.5333
uM
 48
hr
 1 1518 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 134.70 NGFR rabbit IgG(H+L) / donkey NGFR 987.13
WM115-B1-rep1
E4
WM-115 Vemurafenib Resveratrol 1.0000 0.5333
uM
 48
hr
 1 1308 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 126.81 NGFR rabbit IgG(H+L) / donkey NGFR 1113.32
WM115-B1-rep1
E5
WM-115 Vemurafenib Daminozide 1.0000 0.5333
uM
 48
hr
 1 1466 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 136.14 NGFR rabbit IgG(H+L) / donkey NGFR 1015.55
WM115-B1-rep1
E6
WM-115 Vemurafenib I-BET 1.0000 0.5333
uM
 48
hr
 1 1388 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 131.94 NGFR rabbit IgG(H+L) / donkey NGFR 759.27
WM115-B1-rep1
E7
WM-115 Vemurafenib Epigallocatechin gallate 1.0000 0.5333
uM
 48
hr
 1 1363 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 136.88 NGFR rabbit IgG(H+L) / donkey NGFR 1138.47
WM115-B1-rep1
E8
WM-115 Vemurafenib Decitabine 1.0000 0.5333
uM
 48
hr
 1 1312 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 150.52 NGFR rabbit IgG(H+L) / donkey NGFR 949.09
WM115-B1-rep1
F2
WM-115 Vemurafenib Garcinol 1.0000 0.5333
uM
 48
hr
 1 1246 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 139.16 NGFR rabbit IgG(H+L) / donkey NGFR 1098.79
WM115-B1-rep1
F3
WM-115 Vemurafenib Pracinostat 1.0000 0.5333
uM
 48
hr
 1 1333 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 127.90 NGFR rabbit IgG(H+L) / donkey NGFR 666.71
WM115-B1-rep1
F4
WM-115 Vemurafenib BIX-01294 1.0000 0.5333
uM
 48
hr
 1 1331 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 172.39 NGFR rabbit IgG(H+L) / donkey NGFR 974.77
WM115-B1-rep1
F5
WM-115 Vemurafenib Methylstat 1.0000 0.5333
uM
 48
hr
 1 1289 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 122.93 NGFR rabbit IgG(H+L) / donkey NGFR 1114.18
WM115-B1-rep1
F6
WM-115 Vemurafenib I-BET151 1.0000 0.5333
uM
 48
hr
 1 1213 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 125.34 NGFR rabbit IgG(H+L) / donkey NGFR 683.96
WM115-B1-rep1
F7
WM-115 Vemurafenib CX-5461 1.0000 0.5333
uM
 48
hr
 1 1376 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 121.03 NGFR rabbit IgG(H+L) / donkey NGFR 1089.00
WM115-B1-rep1
F8
WM-115 Vemurafenib RG-108 1.0000 0.5333
uM
 48
hr
 1 1156 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 157.47 NGFR rabbit IgG(H+L) / donkey NGFR 1016.92
WM115-B1-rep1
G2