Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 793 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 260.38 NGFR rabbit IgG(H+L) / donkey NGFR 122.78
Colo858-A2-rep1
H8
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 977 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 344.61 NGFR rabbit IgG(H+L) / donkey NGFR 118.55
Colo858-A2-rep1
H9
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 988 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 341.76 NGFR rabbit IgG(H+L) / donkey NGFR 146.13
Colo858-A2-rep2
A10
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1172 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 351.28 NGFR rabbit IgG(H+L) / donkey NGFR 140.37
Colo858-A2-rep2
A11
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1033 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 359.83 NGFR rabbit IgG(H+L) / donkey NGFR 196.89
Colo858-A2-rep2
A2
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 908 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 313.42 NGFR rabbit IgG(H+L) / donkey NGFR 130.36
Colo858-A2-rep2
A3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 811 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 338.76 NGFR rabbit IgG(H+L) / donkey NGFR 129.39
Colo858-A2-rep2
A4
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1117 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 406.39 NGFR rabbit IgG(H+L) / donkey NGFR 145.48
Colo858-A2-rep2
A5
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 923 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 349.90 NGFR rabbit IgG(H+L) / donkey NGFR 131.92
Colo858-A2-rep2
A6
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 851 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 376.95 NGFR rabbit IgG(H+L) / donkey NGFR 149.87
Colo858-A2-rep2
A7
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 972 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 397.01 NGFR rabbit IgG(H+L) / donkey NGFR 132.49
Colo858-A2-rep2
A8
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 965 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 322.70 NGFR rabbit IgG(H+L) / donkey NGFR 158.50
Colo858-A2-rep2
A9
COLO 858 Vemurafenib Olaparib 0.3160 2.6667
uM
 48
hr
 2 1429 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 244.86 NGFR rabbit IgG(H+L) / donkey NGFR 158.62
Colo858-A2-rep2
B2
COLO 858 Vemurafenib Iniparib 0.3160 2.6667
uM
 48
hr
 2 1619 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 309.31 NGFR rabbit IgG(H+L) / donkey NGFR 133.32
Colo858-A2-rep2
B3
COLO 858 Vemurafenib CTB 0.3160 2.6667
uM
 48
hr
 2 1525 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 264.79 NGFR rabbit IgG(H+L) / donkey NGFR 156.84
Colo858-A2-rep2
B4
COLO 858 Vemurafenib Rocilinostat 0.3160 2.6667
uM
 48
hr
 2 596 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 175.51 NGFR rabbit IgG(H+L) / donkey NGFR 157.64
Colo858-A2-rep2
B5
COLO 858 Vemurafenib GSK-J1 0.3160 2.6667
uM
 48
hr
 2 1655 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 349.58 NGFR rabbit IgG(H+L) / donkey NGFR 139.46
Colo858-A2-rep2
B6
COLO 858 Vemurafenib PFI-1 0.3160 2.6667
uM
 48
hr
 2 1014 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 145.85 NGFR rabbit IgG(H+L) / donkey NGFR 19.30
Colo858-A2-rep2
B7
COLO 858 Vemurafenib UNC669 0.3160 2.6667
uM
 48
hr
 2 1750 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 342.24 NGFR rabbit IgG(H+L) / donkey NGFR 153.77
Colo858-A2-rep2
B8
COLO 858 Vemurafenib Veliparib 0.3160 2.6667
uM
 48
hr
 2 1852 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 303.91 NGFR rabbit IgG(H+L) / donkey NGFR 148.05
Colo858-A2-rep2
C2
COLO 858 Vemurafenib Rucaparib 0.3160 2.6667
uM
 48
hr
 2 2191 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 274.88 NGFR rabbit IgG(H+L) / donkey NGFR 168.71
Colo858-A2-rep2
C3
COLO 858 Vemurafenib Belinostat 0.3160 2.6667
uM
 48
hr
 2 165 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 207.48 NGFR rabbit IgG(H+L) / donkey NGFR 20.78
Colo858-A2-rep2
C4
COLO 858 Vemurafenib Selisistat 0.3160 2.6667
uM
 48
hr
 2 2435 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 300.72 NGFR rabbit IgG(H+L) / donkey NGFR 104.24
Colo858-A2-rep2
C5
COLO 858 Vemurafenib GSK-J2 0.3160 2.6667
uM
 48
hr
 2 2739 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 331.72 NGFR rabbit IgG(H+L) / donkey NGFR 152.80
Colo858-A2-rep2
C6
COLO 858 Vemurafenib (+)-JQ1 0.3160 2.6667
uM
 48
hr
 2 1599 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 129.86 NGFR rabbit IgG(H+L) / donkey NGFR 11.70
Colo858-A2-rep2
C7