Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 295.05 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 118.16 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 243.87
A375-P10
F10
A-375 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 189.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 109.08 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 210.36
A375-P10
F11
A-375 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 508.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 149.21 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 230.58
A375-P10
F2
A-375 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 480.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 146.43 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 197.62
A375-P10
F3
A-375 Vemurafenib Pictilisib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 615.17 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 164.82 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 274.56
A375-P10
F4
A-375 Vemurafenib Pictilisib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 502.35 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 148.59 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 207.48
A375-P10
F5
A-375 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 774.02 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 199.06 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 258.33
A375-P10
F6
A-375 Vemurafenib AZD8055 0.316 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 847.30 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 216.14 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 262.95
A375-P10
F7
A-375 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 360.17 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 136.77 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 98.55
A375-P10
F8
A-375 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 418.56 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 149.33 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 77.17
A375-P10
F9
A-375 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 172.81 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 93.73 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 207.21
A375-P10
G10
A-375 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 172.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 95.77 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 180.14
A375-P10
G11
A-375 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 383.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 116.42 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 206.01
A375-P10
G2
A-375 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 482.44 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 150.21 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 196.07
A375-P10
G3
A-375 Vemurafenib Pictilisib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 539.79 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 172.95 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 264.00
A375-P10
G4
A-375 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 575.33 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 188.67 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 212.07
A375-P10
G5
A-375 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 809.04 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 194.24 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 290.26
A375-P10
G6
A-375 Vemurafenib AZD8055 1.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 784.40 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 188.16 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 239.79
A375-P10
G7
A-375 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 283.15 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 118.66 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 82.01
A375-P10
G8
A-375 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 349.48 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 133.72 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 67.57
A375-P10
G9
A-375 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2440.77 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 511.76 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 8412.88
A375-P2
B10
A-375 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1854.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 413.60 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 7390.58
A375-P2
B11
A-375 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3036.79 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 535.40 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 6341.83
A375-P2
B2
A-375 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2927.48 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 487.18 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 5940.11
A375-P2
B3
A-375 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 466.25 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 160.61 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1844.28
A375-P2
B4