Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 380.71 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 138.61 NGFR rabbit IgG(H+L) / donkey NGFR 692.41
A375-P7
G10
A-375 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 295.35 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 110.52 NGFR rabbit IgG(H+L) / donkey NGFR 408.50
A375-P7
G11
A-375 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 536.34 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 138.84 NGFR rabbit IgG(H+L) / donkey NGFR 269.68
A375-P7
G2
A-375 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 571.55 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 140.90 NGFR rabbit IgG(H+L) / donkey NGFR 209.37
A375-P7
G3
A-375 Vemurafenib Pictilisib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 721.86 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 169.72 NGFR rabbit IgG(H+L) / donkey NGFR 737.87
A375-P7
G4
A-375 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 742.66 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 173.25 NGFR rabbit IgG(H+L) / donkey NGFR 428.69
A375-P7
G5
A-375 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 953.39 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 203.29 NGFR rabbit IgG(H+L) / donkey NGFR 530.58
A375-P7
G6
A-375 Vemurafenib AZD8055 1.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 987.57 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 184.95 NGFR rabbit IgG(H+L) / donkey NGFR 465.50
A375-P7
G7
A-375 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 457.50 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 119.63 NGFR rabbit IgG(H+L) / donkey NGFR 310.49
A375-P7
G8
A-375 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 562.14 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 172.19 NGFR rabbit IgG(H+L) / donkey NGFR 243.97
A375-P7
G9
A-375 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 990.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 214.46 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 6076.04
A375-P8
B10
A-375 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 588.47 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 186.71 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 6337.02
A375-P8
B11
A-375 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2353.05 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 495.34 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 7462.55
A375-P8
B2
A-375 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2384.48 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 478.03 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 7901.41
A375-P8
B3
A-375 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2012.07 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 413.37 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 5072.92
A375-P8
B4
A-375 Vemurafenib Pictilisib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1407.94 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 336.02 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 2951.79
A375-P8
B5
A-375 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1464.57 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 324.97 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 974.92
A375-P8
B6
A-375 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1446.04 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 307.33 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 471.65
A375-P8
B7
A-375 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2070.79 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 394.91 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 6240.01
A375-P8
B8
A-375 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1789.77 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 386.63 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 6862.16
A375-P8
B9
A-375 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 368.02 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 126.53 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 2094.50
A375-P8
C10
A-375 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 227.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 99.02 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1461.45
A375-P8
C11
A-375 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 593.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 155.33 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1088.62
A375-P8
C2
A-375 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 546.78 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 155.03 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 803.15
A375-P8
C3
A-375 Vemurafenib Pictilisib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 751.88 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 189.89 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 843.51
A375-P8
C4