Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 93.37 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 114.75 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 739.65
Colo858-P9
G10
COLO 858 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 9.05 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 112.47 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 742.22
Colo858-P9
G11
COLO 858 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 509.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 177.66 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 607.02
Colo858-P9
G2
COLO 858 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 539.47 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 192.62 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 415.11
Colo858-P9
G3
COLO 858 Vemurafenib Pictilisib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 469.06 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 185.49 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 541.20
Colo858-P9
G4
COLO 858 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 367.86 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 178.35 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 482.06
Colo858-P9
G5
COLO 858 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 449.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 183.61 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 748.66
Colo858-P9
G6
COLO 858 Vemurafenib AZD8055 1.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 345.85 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 138.16 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 741.60
Colo858-P9
G7
COLO 858 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 410.15 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 169.53 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 360.95
Colo858-P9
G8
COLO 858 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 392.22 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 165.29 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 238.96
Colo858-P9
G9
WM-115 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 734.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 203.21 NGFR rabbit IgG(H+L) / donkey NGFR 624.02
WM115-P1
B10
WM-115 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 554.72 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 184.01 NGFR rabbit IgG(H+L) / donkey NGFR 580.80
WM115-P1
B11
WM-115 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1336.19 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 271.63 NGFR rabbit IgG(H+L) / donkey NGFR 1363.41
WM115-P1
B2
WM-115 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1023.11 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 238.66 NGFR rabbit IgG(H+L) / donkey NGFR 1240.61
WM115-P1
B3
WM-115 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 498.94 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 138.13 NGFR rabbit IgG(H+L) / donkey NGFR 2782.79
WM115-P1
B4
WM-115 Vemurafenib Trametinib 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 485.80 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 144.44 NGFR rabbit IgG(H+L) / donkey NGFR 2728.27
WM115-P1
B5
WM-115 Vemurafenib Defactinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 789.32 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 204.73 NGFR rabbit IgG(H+L) / donkey NGFR 834.68
WM115-P1
B6
WM-115 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 684.44 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 192.63 NGFR rabbit IgG(H+L) / donkey NGFR 721.67
WM115-P1
B7
WM-115 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 768.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 194.55 NGFR rabbit IgG(H+L) / donkey NGFR 966.13
WM115-P1
B8
WM-115 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 400.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 163.70 NGFR rabbit IgG(H+L) / donkey NGFR 1013.88
WM115-P1
B9
WM-115 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 521.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 144.36 NGFR rabbit IgG(H+L) / donkey NGFR 1045.50
WM115-P1
C10
WM-115 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 438.37 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 136.41 NGFR rabbit IgG(H+L) / donkey NGFR 1154.86
WM115-P1
C11
WM-115 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 785.29 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 168.61 NGFR rabbit IgG(H+L) / donkey NGFR 1928.35
WM115-P1
C2
WM-115 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 677.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 148.62 NGFR rabbit IgG(H+L) / donkey NGFR 2020.98
WM115-P1
C3
WM-115 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 452.41 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 133.24 NGFR rabbit IgG(H+L) / donkey NGFR 3213.79
WM115-P1
C4