Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 509.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 173.36 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 192.18
Colo858-P3
F10
COLO 858 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 733.06 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 204.44 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 138.01
Colo858-P3
F11
COLO 858 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1053.34 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 226.13 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 692.16
Colo858-P3
F2
COLO 858 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 986.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 219.91 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 678.93
Colo858-P3
F3
COLO 858 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 401.48 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 130.71 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 984.71
Colo858-P3
F4
COLO 858 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 447.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 164.60 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 948.70
Colo858-P3
F5
COLO 858 Vemurafenib Defactinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 471.43 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 180.67 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 528.26
Colo858-P3
F6
COLO 858 Vemurafenib Defactinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 394.56 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 165.19 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 518.28
Colo858-P3
F7
COLO 858 Vemurafenib PF562271 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 391.70 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 155.91 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 642.08
Colo858-P3
F8
COLO 858 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 465.90 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 163.37 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 505.86
Colo858-P3
F9
COLO 858 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 600.35 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 183.29 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 209.16
Colo858-P3
G10
COLO 858 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 499.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 227.65 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 121.00
Colo858-P3
G11
COLO 858 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 616.34 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 167.38 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 727.22
Colo858-P3
G2
COLO 858 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 605.41 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 174.89 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 758.99
Colo858-P3
G3
COLO 858 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 430.29 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 144.13 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 981.08
Colo858-P3
G4
COLO 858 Vemurafenib Trametinib 1.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 538.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 151.96 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 869.75
Colo858-P3
G5
COLO 858 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 459.68 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 183.60 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 594.63
Colo858-P3
G6
COLO 858 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 408.70 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 145.99 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 487.91
Colo858-P3
G7
COLO 858 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 407.94 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 146.43 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 634.29
Colo858-P3
G8
COLO 858 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 407.42 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 150.30 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 431.83
Colo858-P3
G9
COLO 858 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3881.18 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 466.69 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 124.41
Colo858-P4
B10
COLO 858 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3490.89 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 412.36 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 99.67
Colo858-P4
B11
COLO 858 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2319.68 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 292.19 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 121.25
Colo858-P4
B2
COLO 858 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2265.01 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 284.02 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 113.58
Colo858-P4
B3
COLO 858 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 465.16 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 150.80 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 405.55
Colo858-P4
B4