Highly-multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the MCF 10A breast cell line treated with EGF or four kinase inhibitors - Dataset (ID:20267)
- Detail
- Small Molecules Studied
- Cell Lines Studied
- Proteins Studied
- Antibodies Studied
- Other Reagents Studied
- Results
Antibodies Studied
Download: Excel (XLSX), Comma-separated (CSV)
| HMS LINCS ID | Name | Alternative Names | Clone Name | LINCS ID | Resource Identification Initiative ID | Nominal Protein Target HMS LINCS ID(s) |
|---|---|---|---|---|---|---|
| 80001-1 | H3 (pS10) | Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #3475 | D2C8 | AB_10694639 | ||
| 80002-2 | p21 / Alexa Fluor 647 | p21 Waf1/Cip1 (12D1) Rabbit mAb (Alexa Fluor® 647 Conjugate) #8587 | 12D1 | AB_10892861 | ||
| 80003-1 | KI-67 / clone D3B5 | Ki-67 (D3B5) Rabbit mAb (Alexa Fluor® 488 Conjugate) #11882 | D3B5 | |||
| 80004-1 | Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 | Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #8957 | D20B12 | |||
| 80005-1 | S6 (pS235; pS236) / Alexa Fluor 647 | Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #4851 | D57.2.2E | AB_916160 | ||
| 80006-1 | S6 (pS240; pS244) / Alexa Fluor 488 | Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP®Rabbit mAb (Alexa Fluor® 488 Conjugate) #5018 | D68F8 | AB_10695861 | ||
| 80007-1 | rabbit IgG(H+L) / goat / cross-adsorbed | Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate | AB_143165 | |||
| 80008-1 | EGFR | EGF Receptor (D38B1) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #5616 | D38B1 | AB_10691853 | ||
| 80011-1 | PCNA | PCNA (PC10) Mouse mAb (Alexa Fluor® 488 Conjugate) #8580 | PC10 | AB_11178664 | ||
| 80012-1 | ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) / Alexa Fluor 488 | Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #4344 | D13.14.4E | AB_2139960 | ||
| 80013-1 | Keratin | Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 555 Conjugate) #3478 | C11 | AB_10829040 | ||
| 80016-1 | Beta-actin | β-Actin (13E5) Rabbit mAb (Alexa Fluor® 555 Conjugate) #8046 | 13E5 | AB_11179208 | ||
| 80022-1 | p53 | p53 Antibody (DO-1): sc-126 | DO-1 | AB_628082 | ||
| 80024-1 | mouse IgG(H+L) / goat | Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 647 conjugate | AB_10564125 | |||
| 80025-1 | Aurora kinase A (pT288); Aurora kinase B (pT232); Aurora kinase C (pT198) | Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (D13A11) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #13464 | D13A11 | |||
| 80026-1 | S6 | S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 555 Conjugate) #6989 | 54D2 | AB_10828226 | ||
| 80027-1 | VEGFR-2 | VEGF Receptor 2 (D5B1) Rabbit mAb (Alexa Fluor® 555 Conjugate) #12872 | D5B1 | |||
| 80028-1 | PARP-1 (D214-cleaved) | Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #6894 | D64E10 | AB_10830735 | ||
| 80029-1 | p27Kip1 / clone Y236 | Anti-p27 KIP 1 antibody [Y236] (Alexa Fluor® 647) (ab194234) | Y236 | |||
| 80030-1 | H2A.X (pS139) / clone 20E3 / Alexa Fluor 647 | Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (Alexa Fluor® 647 Conjugate) #9720 | 20E3 | AB_10692910 | ||
| 80031-1 | AKT | Akt (pan) (C67E7) Rabbit mAb (Alexa Fluor® 647 Conjugate) #5186 | C67E7 | AB_10695877 | ||
| 80032-1 | mTOR | mTOR (7C10) Rabbit mAb (Alexa Fluor® 647 Conjugate) #5048 | 7C10 | AB_10828101 | ||
| 80033-1 | Vimentin | Vimentin (D21H3) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #9856 | D21H3 | AB_10834530 | ||
| 80034-1 | NF-kB p65 | Anti-NF-kB p65 antibody [E379] (Alexa Fluor® 647) (ab190589) | E379 | |||
| 80035-1 | FOXO3a | FoxO3a (75D8) Rabbit mAb #2497 | 75D8 | AB_836876 |