Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 253.8 5
0020-0107
 189.04 107.38 386 77.50 0.808
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 421.2 5
0021-0106
 912.13 105.94 263 75.50 0.580
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 283.2 5
0022-0110
 20.05 109.88 419 79.74 0.828
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 421.7 5
0023-0113
 684.55 113.61 430 78.08 0.886
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 848.3 5
0024-0134
 634.63 132.56 822 126.37 0.647
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 256.7 5
0025-0135
 217.50 135.58 433 79.84 0.854
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 266.5 5
0026-0138
 151.19 137.96 392 74.67 0.884
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 291.3 5
0027-0139
 694.30 139.48 432 78.91 0.872
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 418.2 5
0028-0138
 506.42 138.97 366 71.26 0.906
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 468.0 5
0029-0153
 739.43 153.21 377 71.01 0.939
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 1521.7 5
0030-0155
 266.99 155.23 390 74.08 0.893
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 299.5 5
0031-0158
 364.21 158.24 390 74.43 0.885
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 280.2 5
0032-0157
 789.19 157.46 339 69.84 0.873
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 486.8 5
0033-0161
 428.45 161.27 415 79.15 0.832
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 434.2 5
0034-0162
 606.91 162.23 471 85.98 0.801
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 280.4 5
0035-0164
 102.21 164.37 385 74.91 0.862
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 304.9 5
0036-0170
 393.74 169.85 402 75.84 0.878
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 598.2 5
0037-0173
 45.29 173.71 724 105.50 0.817
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 594.0 5
0038-0176
 559.97 176.57 534 125.44 0.426
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 226.5 5
0039-0182
 182.79 182.45 382 74.33 0.869
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 266.2 5
0040-0184
 824.00 184.55 431 79.84 0.850
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 252.9 5
0041-0189
 630.02 190.57 822 113.54 0.801
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 229.3 5
0042-0185
 228.36 183.91 443 87.05 0.735
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 396.8 5
0043-0202
 452.20 201.93 838 110.57 0.861
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 356.3 5
0044-0195
 279.94 195.23 410 77.01 0.869