Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 255.7 4
0193-0688
 31.90 688.55 387 75.60 0.851
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 224.3 4
0194-0700
 913.09 698.64 422 81.15 0.805
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 333.2 4
0195-0699
 177.40 699.16 425 77.01 0.900
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 242.5 4
0196-0706
 896.96 706.45 370 76.67 0.791
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 414.2 4
0197-0705
 451.24 705.04 393 73.01 0.926
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 316.4 4
0198-0706
 841.19 706.47 391 73.84 0.901
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 1480.2 5
0001-0036
 912.92 36.08 697 97.74 0.917
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 375.1 5
0002-0037
 400.71 36.02 190 56.53 0.747
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 281.6 5
0003-0042
 383.54 44.47 533 95.15 0.740
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 295.2 5
0004-0043
 672.96 42.74 427 77.84 0.886
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 381.0 5
0005-0047
 55.66 46.50 612 93.98 0.871
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 1381.4 5
0006-0047
 87.28 46.68 387 97.15 0.515
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 522.3 5
0007-0044
 186.17 44.47 395 73.84 0.910
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 307.6 5
0008-0047
 226.42 47.21 458 83.15 0.832
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 380.4 5
0009-0048
 129.84 47.85 425 77.01 0.900
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 386.1 5
0010-0050
 790.39 50.36 408 77.74 0.848
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 2886.4 5
0011-0051
 101.71 52.26 139 54.53 0.587
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 261.8 5
0012-0057
 312.17 57.19 430 79.26 0.860
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 254.2 5
0013-0068
 489.97 68.32 441 79.50 0.877
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 297.5 5
0014-0073
 372.57 73.36 407 74.43 0.923
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 280.0 5
0015-0074
 412.97 74.21 406 77.50 0.849
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 342.5 5
0016-0082
 710.92 82.13 436 77.01 0.924
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 318.5 5
0017-0089
 155.98 89.08 370 72.43 0.886
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 319.1 5
0018-0098
 744.01 98.60 365 71.26 0.903
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 312.6 5
0019-0101
 112.51 100.66 349 70.43 0.884