Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 246.5 8
0099-0464
 52.51 464.29 258 63.01 0.817
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 282.4 8
0100-0475
 743.13 475.27 277 63.01 0.877
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 276.5 8
0101-0501
 414.64 501.00 335 69.60 0.869
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 263.3 8
0102-0507
 566.44 507.64 333 69.60 0.864
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 249.9 8
0103-0514
 374.99 514.14 306 65.94 0.884
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 220.6 8
0104-0513
 334.32 513.20 159 48.53 0.848
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 238.5 8
0105-0524
 327.91 524.49 200 53.94 0.864
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 299.1 8
0106-0524
 268.31 524.45 261 62.53 0.839
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 312.2 8
0107-0531
 853.53 531.31 273 60.77 0.929
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 259.4 8
0108-0535
 604.96 534.87 366 72.18 0.883
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 257.5 8
0109-0571
 800.98 571.40 335 71.01 0.835
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 296.8 8
0110-0569
 210.82 568.62 319 65.60 0.932
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 303.4 8
0111-0577
 472.98 576.95 286 63.60 0.889
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 244.8 8
0112-0581
 98.85 581.31 272 62.77 0.868
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 208.7 8
0113-0580
 145.09 579.96 365 73.01 0.860
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 269.4 8
0114-0590
 882.83 590.92 326 71.26 0.807
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 245.0 8
0115-0597
 725.29 596.96 315 68.18 0.851
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 253.4 8
0116-0597
 624.95 597.75 362 73.26 0.848
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 324.8 8
0117-0611
 935.55 610.24 234 62.43 0.755
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 207.1 8
0118-0618
 164.12 619.05 342 69.84 0.881
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 288.2 8
0119-0619
 390.63 619.56 358 72.18 0.863
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 263.4 8
0120-0624
 311.05 624.35 393 74.53 0.889
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 240.8 8
0121-0633
 257.00 633.60 354 72.18 0.854
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 350.0 8
0122-0629
 887.85 629.27 229 59.36 0.817
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 580.3 8
0123-0631
 485.97 631.58 369 71.60 0.905