Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 513.8 5
0179-0654
490.62 655.31 427 97.40 0.566
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 254.0 5
0180-0654
290.92 654.14 391 75.26 0.868
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 267.2 5
0181-0658
122.51 658.29 430 78.08 0.886
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 293.8 5
0182-0660
943.92 659.79 269 61.36 0.898
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 232.0 5
0183-0665
450.32 665.15 408 76.67 0.872
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 233.1 5
0184-0666
711.38 665.77 407 78.08 0.839
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 217.6 5
0185-0665
388.66 665.79 387 76.08 0.840
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 215.1 5
0186-0667
530.37 666.86 408 77.74 0.848
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 222.8 5
0187-0670
36.41 670.08 383 75.50 0.844
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 226.4 5
0188-0670
867.56 669.86 372 72.43 0.891
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 240.7 5
0189-0687
601.90 686.94 404 75.84 0.883
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 222.7 5
0190-0689
805.54 688.90 400 76.67 0.855
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 227.5 5
0191-0691
754.41 691.52 398 75.01 0.889
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 194.5 5
0192-0694
706.03 694.46 414 77.50 0.866
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 188.9 5
0193-0695
182.32 695.39 392 74.67 0.884
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 211.0 5
0194-0698
408.47 698.36 394 76.08 0.855
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 170.0 5
0195-0701
18.07 700.07 363 77.40 0.761
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 232.6 5
0196-0700
311.11 700.68 390 74.67 0.879
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 207.2 5
0197-0707
882.43 706.19 366 72.81 0.868
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 237.6 6
0001-0039
609.10 39.78 429 78.67 0.871
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 409.9 6
0002-0037
360.26 37.48 370 71.60 0.907
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 238.4 6
0003-0043
130.87 43.35 415 76.67 0.887
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 233.5 6
0004-0044
937.28 43.85 447 83.26 0.810
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 260.1 6
0005-0046
666.80 46.34 393 73.01 0.926
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 215.7 6
0006-0051
182.30 50.93 439 79.84 0.865