Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 240.2 4
0127-0431
777.43 430.82 454 83.15 0.825
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 250.2 4
0128-0436
705.74 437.07 413 81.98 0.772
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 249.6 4
0129-0435
319.58 435.80 410 76.08 0.890
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 261.8 4
0130-0446
599.20 446.02 744 100.81 0.920
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 404.0 4
0131-0448
163.99 448.51 430 78.08 0.886
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 261.3 4
0132-0456
276.89 456.42 424 78.08 0.874
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 267.2 4
0133-0459
905.75 457.52 380 75.98 0.827
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 270.9 4
0134-0468
888.60 469.74 418 79.74 0.826
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 333.4 4
0135-0474
695.70 474.53 764 106.23 0.851
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 241.7 4
0136-0477
370.65 476.79 426 80.81 0.820
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 254.7 4
0137-0481
112.68 481.24 401 78.33 0.821
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 265.7 4
0138-0488
213.74 488.34 406 78.67 0.824
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 231.2 4
0139-0486
603.59 485.86 424 78.91 0.856
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 251.6 4
0140-0492
76.49 492.01 442 82.33 0.820
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 239.5 4
0141-0490
307.38 490.29 448 81.50 0.848
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 316.1 4
0142-0495
355.80 495.48 508 86.57 0.852
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 255.1 4
0143-0498
530.91 497.54 426 78.43 0.870
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 270.3 4
0144-0508
936.11 508.18 411 76.43 0.884
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 355.8 4
0145-0516
640.42 515.71 391 73.60 0.907
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 237.5 4
0146-0518
385.44 518.92 445 83.98 0.793
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 227.7 4
0147-0526
569.04 526.32 431 80.33 0.839
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 251.6 4
0148-0525
428.94 525.80 418 80.33 0.814
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 216.5 4
0149-0528
701.21 528.02 431 79.50 0.857
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 331.0 4
0150-0535
118.78 535.26 432 80.91 0.829
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 279.2 4
0151-0535
367.11 534.85 449 81.74 0.844