Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 221.7 8
0085-0284
185.71 284.27 400 77.74 0.832
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 318.6 8
0086-0282
884.17 282.00 325 67.01 0.909
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 212.7 8
0087-0284
913.06 284.55 406 75.84 0.887
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 286.8 8
0088-0285
118.78 285.70 421 81.40 0.799
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 217.5 8
0089-0284
691.85 283.76 461 83.36 0.834
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 205.5 8
0090-0286
590.33 286.02 441 83.40 0.797
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 473.3 8
0091-0289
62.19 290.24 495 87.05 0.821
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 289.0 8
0092-0289
215.23 288.87 428 76.67 0.915
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 256.0 8
0093-0292
545.64 292.36 775 106.23 0.863
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 267.1 8
0094-0292
733.36 292.72 814 108.57 0.868
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 211.2 8
0095-0292
385.56 292.55 865 120.81 0.745
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 250.7 8
0096-0294
505.09 292.74 453 88.47 0.727
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 181.9 8
0097-0301
945.77 302.32 413 79.26 0.826
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 232.2 8
0098-0321
99.54 320.91 400 76.91 0.850
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 236.3 8
0099-0321
502.87 321.41 431 77.84 0.894
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 240.8 8
0100-0319
659.31 318.85 429 79.26 0.858
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 241.5 8
0101-0336
890.29 336.90 826 119.68 0.725
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 209.8 8
0102-0329
912.12 328.16 412 82.33 0.764
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 210.1 8
0103-0332
28.83 332.23 415 79.15 0.832
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 233.7 8
0104-0336
249.69 335.89 411 79.74 0.812
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 191.8 8
0105-0334
844.38 334.08 456 79.01 0.918
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 230.1 8
0106-0339
425.15 339.81 395 77.60 0.824
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 237.5 8
0107-0340
652.93 341.37 469 84.91 0.817
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 225.2 8
0108-0342
342.76 342.06 413 78.67 0.839
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 244.4 8
0109-0341
386.82 341.56 467 83.50 0.842