Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 246.8 4
0042-0306
 120.00 305.13 301 67.26 0.836
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 272.7 4
0043-0306
 917.57 306.35 266 63.01 0.842
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 330.9 4
0044-0307
 538.95 307.10 280 61.60 0.927
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 364.6 4
0045-0309
 667.97 309.75 240 61.60 0.795
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 513.6 4
0046-0306
 58.29 305.81 108 71.26 0.267
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 281.3 4
0047-0311
 377.59 311.42 323 68.77 0.858
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 313.0 4
0048-0325
 400.90 325.45 277 65.60 0.809
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 273.0 4
0049-0329
 359.73 329.52 366 72.43 0.877
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 249.7 4
0050-0341
 175.33 341.14 295 67.36 0.817
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 269.6 4
0051-0338
 296.96 338.14 314 68.43 0.843
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 244.8 4
0052-0335
 911.15 335.22 279 63.36 0.873
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 312.3 4
0053-0339
 454.32 339.21 317 67.60 0.872
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 421.8 4
0054-0340
 751.27 340.20 239 56.53 0.940
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 370.4 4
0055-0347
 199.86 347.24 232 57.36 0.886
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 284.0 4
0056-0348
 898.53 348.06 261 62.18 0.848
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 288.7 4
0057-0353
 925.53 352.89 270 61.94 0.884
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 350.8 4
0058-0370
 645.70 369.78 240 56.53 0.944
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 282.9 4
0059-0374
 765.64 375.08 305 63.60 0.948
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 307.5 4
0060-0377
 230.62 377.83 321 67.84 0.876
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 312.1 4
0061-0375
 75.09 374.82 228 58.87 0.827
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 301.6 4
0062-0397
 347.58 396.96 251 61.01 0.847
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 303.7 4
0063-0400
 196.29 400.36 253 59.11 0.910
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 238.4 4
0064-0399
 845.43 399.14 274 61.11 0.922
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 266.0 4
0065-0405
 765.45 405.15 309 65.60 0.902
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 270.7 4
0066-0407
 710.17 407.47 313 64.43 0.948