Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 281.1 1
0192-0581
 202.82 581.40 407 77.84 0.844
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 281.2 1
0193-0584
 360.11 584.44 373 73.26 0.873
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 327.0 1
0194-0585
 610.58 584.79 382 73.26 0.895
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 288.5 1
0195-0583
 861.48 583.27 393 78.33 0.805
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 283.2 1
0196-0591
 809.80 591.24 406 78.91 0.819
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 268.3 1
0197-0602
 194.05 601.73 235 60.77 0.800
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 223.6 1
0198-0606
 401.04 605.93 421 77.26 0.886
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 223.7 1
0199-0607
 249.23 606.40 392 78.67 0.796
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 249.7 1
0200-0607
 715.39 607.94 388 76.67 0.829
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 220.4 1
0201-0609
 886.12 609.68 379 75.84 0.828
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 358.5 1
0202-0613
 662.08 613.08 400 74.67 0.902
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 228.0 1
0203-0613
 81.87 613.13 432 82.81 0.792
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 311.2 1
0204-0619
 359.78 619.40 408 77.60 0.851
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 248.9 1
0205-0623
 500.22 622.62 416 77.50 0.870
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 233.5 1
0206-0632
 54.26 631.53 380 72.67 0.904
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 203.2 1
0207-0634
 156.18 634.61 438 77.84 0.908
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 266.8 1
0208-0632
 314.53 632.31 371 75.60 0.816
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 262.3 1
0209-0635
 337.73 634.71 468 90.18 0.723
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 210.2 1
0210-0639
 845.81 638.80 759 104.33 0.876
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 221.4 1
0211-0637
 182.83 636.98 452 85.40 0.779
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 242.7 1
0212-0638
 479.84 638.61 466 84.91 0.812
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 255.9 1
0213-0643
 360.80 643.00 528 91.84 0.787
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 308.0 1
0214-0643
 403.72 643.53 391 72.43 0.937
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 263.3 1
0215-0644
 797.68 644.62 380 74.43 0.862
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 384.9 1
0216-0648
 23.17 647.57 369 76.18 0.799