Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 265.6 1
0167-0512
606.62 511.74 393 76.08 0.853
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 315.9 1
0168-0513
745.60 513.60 376 73.01 0.886
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 232.2 1
0169-0513
487.28 512.82 395 76.08 0.857
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 192.4 1
0170-0517
895.51 516.63 393 75.50 0.866
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 232.7 1
0171-0521
326.02 521.07 373 71.60 0.914
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 310.5 1
0172-0523
118.03 523.23 386 72.18 0.931
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 292.3 1
0173-0525
289.17 524.87 410 76.67 0.877
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 241.1 1
0174-0525
420.66 525.54 395 76.08 0.857
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 246.0 1
0175-0528
648.78 528.16 396 74.43 0.898
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 307.6 1
0176-0530
163.13 530.49 391 74.67 0.881
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 427.0 1
0177-0530
771.34 530.25 390 73.01 0.919
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 268.0 1
0178-0533
199.57 533.05 381 73.60 0.884
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 222.7 1
0179-0540
385.59 540.73 417 78.91 0.842
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 261.1 1
0180-0539
543.36 539.82 398 75.84 0.870
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 265.5 1
0181-0547
897.72 547.42 433 85.40 0.746
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 232.6 1
0182-0544
40.20 544.43 338 70.67 0.850
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 357.3 1
0183-0548
473.27 547.35 620 101.40 0.758
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 492.6 1
0184-0562
275.66 562.66 620 92.91 0.903
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 312.5 1
0185-0571
557.83 571.47 817 113.78 0.793
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 252.0 1
0186-0565
658.42 565.82 393 73.84 0.906
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 339.8 1
0187-0570
94.36 570.37 340 68.18 0.919
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 289.5 1
0188-0570
756.96 569.72 404 76.43 0.869
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 256.1 1
0189-0573
890.54 574.05 415 78.43 0.848
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 375.1 1
0190-0575
718.87 575.35 418 79.15 0.838
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 215.1 1
0191-0584
443.06 584.72 994 128.02 0.762