Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 4258.7 5
0106-0427
 40.59 427.14 247 64.53 0.745
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1078.8 5
0107-0430
 435.55 429.89 458 82.33 0.849
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1735.2 5
0108-0440
 211.05 440.67 269 62.77 0.858
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 343.0 5
0109-0439
 640.00 439.36 249 57.94 0.932
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1458.0 5
0110-0442
 417.16 442.94 262 61.01 0.884
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 320.7 5
0111-0443
 899.55 443.27 301 64.18 0.918
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 236.6 5
0112-0447
 476.08 447.55 363 70.67 0.913
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 555.9 5
0113-0451
 734.64 451.15 297 66.18 0.852
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 266.9 5
0114-0456
 242.30 455.90 332 69.60 0.861
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 331.5 5
0115-0451
 578.34 451.24 303 68.77 0.805
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 729.5 5
0116-0469
 58.94 469.69 252 61.60 0.835
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 354.9 5
0117-0471
 351.25 471.21 327 70.43 0.828
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 3208.0 5
0118-0472
 551.99 471.88 329 67.11 0.918
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2007.3 5
0119-0472
 203.72 471.30 258 61.94 0.845
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1657.9 5
0120-0478
 503.26 478.79 262 67.11 0.731
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 274.5 5
0121-0485
 856.17 484.58 358 83.15 0.651
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 341.3 5
0122-0485
 677.69 485.26 282 61.36 0.941
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 295.6 5
0123-0487
 399.87 488.02 359 73.50 0.835
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 292.5 5
0124-0490
 440.42 490.09 294 65.01 0.874
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 460.8 5
0125-0498
 773.48 496.68 378 75.60 0.831
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 418.7 5
0126-0496
 309.69 496.05 294 69.84 0.757
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 624.8 5
0127-0509
 643.66 509.29 335 73.94 0.770
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 346.9 5
0128-0509
 589.08 509.68 338 69.60 0.877
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 940.9 5
0129-0513
 89.09 513.87 285 64.77 0.854
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 358.1 5
0130-0514
 719.54 514.93 320 72.91 0.756