Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 287.7 3
0112-0630
 903.23 629.42 344 73.50 0.800
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 343.1 3
0113-0631
 686.81 631.00 315 66.43 0.897
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 346.5 3
0114-0638
 664.80 638.76 311 67.60 0.855
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 286.8 3
0115-0642
 347.01 643.02 393 73.01 0.926
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 786.3 3
0116-0644
 17.93 645.04 252 59.36 0.899
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 300.5 3
0117-0648
 121.67 648.11 346 72.18 0.834
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 676.8 3
0118-0651
 291.92 651.05 242 62.77 0.772
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 315.0 3
0119-0654
 805.48 654.07 367 80.57 0.710
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1350.7 3
0120-0652
 309.59 653.07 239 60.77 0.813
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 306.2 3
0121-0658
 176.52 658.47 307 64.77 0.920
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 314.9 3
0122-0658
 839.55 658.18 315 69.84 0.812
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 658.8 3
0123-0665
 557.91 665.19 318 65.60 0.929
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 403.2 3
0124-0672
 147.01 672.49 294 63.60 0.913
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 333.4 3
0125-0682
 690.56 682.16 287 63.60 0.892
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 837.2 3
0126-0686
 535.26 685.35 203 55.21 0.837
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2162.5 3
0127-0686
 26.79 686.38 256 61.11 0.861
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 366.7 3
0128-0694
 675.48 694.25 328 67.60 0.902
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 411.7 3
0129-0694
 879.58 694.71 250 59.94 0.874
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 469.5 3
0130-0704
 182.73 704.55 273 61.60 0.904
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 566.7 3
0131-0705
 571.76 705.50 261 61.94 0.855
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 484.9 4
0001-0029
 171.82 29.32 261 60.77 0.888
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 529.3 4
0002-0029
 195.04 28.79 276 62.28 0.894
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 382.6 4
0003-0032
 144.75 32.73 257 61.70 0.848
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 389.3 4
0004-0037
 371.19 36.91 404 78.43 0.825
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 442.0 4
0005-0064
 872.22 63.25 356 72.43 0.853