Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 401.8 4
0094-0339
696.49 338.86 459 81.01 0.879
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 481.8 4
0095-0341
573.65 341.09 457 84.91 0.797
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 301.1 4
0096-0341
279.50 340.56 475 82.91 0.868
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 383.5 4
0097-0345
217.15 344.72 442 78.43 0.903
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 299.3 4
0098-0350
353.90 350.58 483 87.74 0.788
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 412.8 4
0099-0350
829.44 351.06 433 78.33 0.887
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 396.5 4
0100-0357
882.34 358.36 885 117.54 0.805
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 309.4 4
0101-0353
512.95 353.82 454 80.08 0.890
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 311.1 4
0102-0357
263.78 357.96 479 86.23 0.810
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 344.1 4
0103-0361
645.71 361.63 519 88.67 0.830
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 332.5 4
0104-0364
616.45 364.48 490 86.57 0.822
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 679.1 4
0105-0365
788.10 365.64 480 81.84 0.901
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 283.6 4
0106-0369
420.08 369.56 434 79.26 0.868
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 309.2 4
0107-0378
935.44 378.34 450 80.67 0.869
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 161.4 4
0108-0378
149.62 378.56 429 75.84 0.937
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 307.6 4
0109-0384
640.88 384.35 489 87.60 0.801
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 660.0 4
0110-0384
307.53 384.07 449 79.84 0.885
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 300.6 4
0111-0390
83.47 390.26 414 78.33 0.848
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 2280.5 4
0112-0398
358.78 397.74 826 108.47 0.882
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 379.1 4
0113-0398
798.87 397.96 445 84.33 0.786
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 354.8 4
0114-0402
595.24 401.63 512 86.23 0.865
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1382.9 4
0115-0405
654.01 406.06 567 92.57 0.832
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1659.6 4
0116-0405
260.98 405.31 536 88.57 0.859
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 278.8 4
0117-0411
535.96 411.71 918 120.23 0.798
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 2213.9 4
0118-0415
484.12 415.53 806 109.74 0.841