Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 404.5 4
0069-0263
692.44 264.22 439 79.01 0.884
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 369.0 4
0070-0268
225.86 267.89 430 78.91 0.868
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 462.8 4
0071-0267
259.01 267.57 426 79.50 0.847
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 304.7 4
0072-0273
54.20 273.66 414 80.33 0.806
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 309.9 4
0073-0275
426.18 274.91 483 83.50 0.871
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 433.9 4
0074-0282
565.08 282.42 965 129.78 0.720
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 326.3 4
0075-0274
917.38 275.01 446 79.60 0.885
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 332.8 4
0076-0276
842.17 276.79 446 82.91 0.815
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 245.8 4
0077-0286
325.72 285.68 477 84.91 0.831
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 397.5 4
0078-0291
690.26 290.59 484 87.01 0.803
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 456.2 4
0079-0289
163.66 288.76 423 75.26 0.939
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 381.2 4
0080-0295
69.01 296.21 555 93.01 0.806
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 406.4 4
0081-0298
631.28 298.98 823 111.30 0.835
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 520.8 4
0082-0297
92.75 297.45 410 76.53 0.880
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 321.1 4
0083-0298
486.50 297.74 444 79.50 0.883
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 241.3 4
0084-0296
730.71 296.02 421 77.84 0.873
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1794.1 4
0085-0309
336.67 309.13 741 102.91 0.879
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 304.7 4
0086-0310
792.80 310.62 440 79.26 0.880
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 472.0 4
0087-0314
583.95 314.44 503 91.84 0.749
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 310.3 4
0088-0319
915.92 319.07 927 119.20 0.820
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 330.4 4
0089-0323
526.57 322.61 442 78.08 0.911
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 502.5 4
0090-0329
369.87 328.39 447 80.67 0.863
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 275.3 4
0091-0333
741.86 333.44 431 77.26 0.907
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 265.9 4
0092-0335
405.26 335.05 449 79.50 0.893
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 779.9 4
0093-0338
442.23 337.84 665 97.64 0.877