Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 222.1 5
0027-0316
 61.57 315.91 259 68.53 0.693
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 240.4 5
0028-0324
 909.41 324.06 225 55.11 0.931
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 208.0 5
0029-0351
 34.16 350.35 160 51.11 0.770
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 203.4 5
0030-0356
 852.99 356.90 319 68.18 0.862
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 213.5 5
0031-0357
 43.91 357.60 134 43.46 0.892
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 218.2 5
0032-0370
 916.33 370.90 242 64.53 0.730
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 240.4 5
0033-0388
 478.45 387.91 263 61.94 0.861
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 340.5 5
0034-0396
 394.79 396.25 187 51.94 0.871
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 276.9 5
0035-0412
 692.19 411.81 233 56.28 0.924
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 263.6 5
0036-0419
 429.87 419.08 156 58.77 0.568
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 221.1 5
0037-0425
 50.46 424.75 261 63.60 0.811
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 250.7 5
0038-0484
 95.58 484.30 269 62.18 0.874
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 324.2 5
0039-0483
 930.96 483.42 201 53.70 0.876
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 259.5 5
0040-0494
 633.21 493.73 212 62.28 0.687
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 265.2 5
0041-0497
 769.80 497.55 253 61.70 0.835
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 220.2 5
0042-0508
 329.53 508.48 329 72.43 0.788
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 248.2 5
0043-0527
 286.51 526.75 220 61.01 0.743
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 267.0 5
0044-0535
 622.69 534.63 187 56.28 0.742
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 224.7 5
0045-0550
 220.89 550.54 202 57.94 0.756
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 222.8 5
0046-0548
 249.45 548.43 172 52.28 0.791
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 306.2 5
0047-0563
 153.70 562.95 110 53.36 0.486
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 247.5 5
0048-0583
 634.62 583.75 264 63.84 0.814
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 286.2 5
0049-0594
 129.69 594.89 191 54.18 0.818
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 213.3 5
0050-0606
 526.68 606.72 254 62.53 0.816
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 219.1 5
0051-0621
 548.39 620.38 261 60.53 0.895