Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1379.2 6
0280-0492
513.77 492.88 252 59.11 0.906
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1305.6 6
0281-0498
48.00 498.20 251 59.94 0.878
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 3437.6 6
0282-0498
852.36 498.53 283 65.60 0.826
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 542.5 6
0283-0500
111.63 500.19 240 58.77 0.873
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1321.9 6
0284-0500
478.43 499.97 207 51.46 0.982
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 2666.3 6
0285-0501
795.41 501.71 210 53.70 0.915
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 3717.1 6
0286-0503
443.46 502.86 393 76.67 0.840
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 4055.6 6
0287-0504
559.57 504.46 323 67.36 0.895
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 2928.1 6
0288-0504
831.86 504.47 290 62.53 0.932
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 854.9 6
0289-0505
333.16 504.68 199 54.87 0.831
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1170.0 6
0290-0509
934.43 509.12 471 88.57 0.755
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1672.7 6
0291-0510
601.38 510.61 350 70.43 0.887
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 806.1 6
0292-0512
911.09 511.98 298 68.18 0.805
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 4534.0 6
0293-0514
516.97 514.15 236 57.70 0.891
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1674.3 6
0294-0515
42.43 515.91 283 62.18 0.920
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 598.8 6
0295-0516
653.99 516.16 302 66.18 0.866
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 958.1 6
0296-0518
896.99 517.89 228 60.77 0.776
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1344.6 6
0297-0518
64.81 518.39 236 56.77 0.920
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1324.1 6
0298-0521
259.18 521.20 288 64.43 0.872
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 3253.7 6
0299-0522
759.26 522.01 321 66.77 0.905
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 515.1 6
0300-0521
695.19 521.83 316 67.84 0.863
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 991.3 6
0301-0524
307.39 524.49 237 63.60 0.736
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 5426.6 6
0302-0524
537.36 523.04 202 57.36 0.772
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 6111.0 6
0303-0526
738.26 525.71 335 67.11 0.935
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 922.9 6
0304-0530
442.40 529.89 215 53.94 0.929