Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 271.7 3
0010-0154
 925.91 153.92 225 57.36 0.859
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 276.5 3
0011-0159
 132.00 159.59 256 62.53 0.823
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 296.7 3
0012-0161
 248.69 161.09 118 40.28 0.914
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 230.0 3
0013-0170
 238.79 170.81 151 46.53 0.877
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 353.0 3
0014-0176
 428.50 176.01 204 58.18 0.757
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 243.6 3
0015-0181
 503.87 181.33 291 66.77 0.820
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 593.8 3
0016-0180
 529.81 180.56 171 50.53 0.842
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 377.8 3
0017-0197
 582.62 198.37 177 56.18 0.705
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 301.5 3
0018-0200
 927.92 200.57 216 59.60 0.764
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 372.7 3
0019-0218
 416.36 218.30 165 50.04 0.828
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 349.5 3
0020-0225
 645.39 226.73 372 80.08 0.729
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 258.5 3
0021-0230
 534.48 231.10 267 63.26 0.839
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 244.6 3
0022-0244
 246.67 242.06 190 58.87 0.689
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 316.7 3
0023-0252
 396.23 252.19 223 54.53 0.943
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 252.2 3
0024-0254
 528.93 254.27 270 65.01 0.803
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 240.2 3
0025-0258
 249.56 257.64 117 41.70 0.846
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 249.8 3
0026-0275
 172.46 274.70 276 67.84 0.754
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 254.3 3
0027-0281
 631.58 281.17 262 59.94 0.916
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 298.0 3
0028-0283
 13.67 282.64 191 56.77 0.745
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 317.6 3
0029-0291
 854.26 290.53 224 59.60 0.792
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 295.9 3
0030-0303
 103.18 303.57 207 57.36 0.791
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 898.8 3
0031-0312
 777.94 311.63 151 51.46 0.717
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 314.1 3
0032-0322
 487.29 322.50 214 56.77 0.834
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 238.3 3
0033-0343
 170.81 343.65 233 58.28 0.862
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 345.6 3
0034-0384
 852.19 384.44 220 55.36 0.902