Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 280.2 2
0017-0511
 49.96 511.64 225 57.70 0.849
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 243.2 2
0018-0515
 114.80 515.24 268 63.60 0.833
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 292.3 2
0019-0613
 133.89 613.28 223 60.53 0.765
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 300.0 2
0020-0618
 504.01 618.58 259 63.36 0.811
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 246.3 2
0021-0635
 637.25 635.23 302 66.53 0.857
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 221.7 2
0022-0639
 285.94 639.91 297 66.18 0.852
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 248.1 2
0023-0644
 591.27 644.02 278 63.70 0.861
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 233.0 2
0024-0650
 538.77 650.29 296 64.53 0.893
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 219.2 2
0025-0654
 224.91 653.80 279 63.70 0.864
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 231.0 2
0026-0669
 100.45 669.38 299 65.36 0.880
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 245.3 2
0027-0672
 582.47 672.12 374 76.08 0.812
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 245.7 2
0028-0672
 305.14 673.35 281 64.53 0.848
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 482.0 2
0029-0686
 316.60 686.53 125 45.11 0.772
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 237.6 2
0030-0691
 559.22 690.33 333 73.01 0.785
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 222.5 2
0031-0697
 615.07 697.26 277 62.18 0.900
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 1260.8 2
0032-0703
 912.12 702.79 115 40.28 0.891
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 306.7 3
0001-0051
 392.71 51.40 201 55.36 0.824
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 290.7 3
0002-0075
 222.13 75.24 244 59.36 0.870
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 327.1 3
0003-0084
 824.96 84.25 264 61.94 0.865
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 257.0 3
0004-0098
 283.01 98.19 236 61.26 0.790
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 287.8 3
0005-0107
 865.02 107.68 209 59.26 0.748
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 291.9 3
0006-0109
 428.96 108.97 213 54.53 0.900
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 298.3 3
0007-0117
 337.10 117.22 177 52.18 0.817
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 303.2 3
0008-0120
 858.06 120.25 224 55.94 0.899
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 391.7 3
0009-0140
 463.15 140.84 127 57.60 0.481