Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 232.0 6
0130-0572
 573.03 573.00 363 76.08 0.788
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 269.9 6
0131-0570
 899.87 570.25 219 55.70 0.887
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 248.1 6
0132-0581
 58.91 581.53 285 62.18 0.926
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 211.1 6
0133-0585
 191.91 585.57 308 69.26 0.807
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 211.8 6
0134-0581
 760.19 580.73 169 51.36 0.805
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 244.6 6
0135-0586
 779.09 586.58 318 73.70 0.736
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 190.8 6
0136-0593
 393.16 593.77 325 68.77 0.864
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 196.9 6
0137-0598
 524.28 598.86 360 74.43 0.817
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 221.1 6
0138-0604
 407.96 603.66 268 60.77 0.912
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 197.0 6
0139-0606
 719.80 606.67 342 71.60 0.838
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 213.0 6
0140-0610
 668.02 609.38 332 71.60 0.814
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 167.0 6
0141-0610
 944.17 610.24 325 67.36 0.900
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 235.7 6
0142-0624
 254.48 624.73 435 81.36 0.826
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 311.7 6
0143-0620
 473.85 620.29 254 59.94 0.888
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 229.3 6
0144-0625
 498.09 624.46 322 69.60 0.835
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 726.8 6
0145-0621
 588.56 622.24 126 47.11 0.713
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 440.1 6
0146-0626
 604.38 625.56 306 69.36 0.799
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 217.1 6
0147-0631
 399.30 631.24 270 60.18 0.937
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 225.2 6
0148-0643
 820.36 643.79 331 69.60 0.859
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 224.4 6
0149-0647
 762.95 647.48 391 74.67 0.881
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 232.1 6
0150-0647
 185.53 647.15 314 69.01 0.828
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 312.5 6
0151-0650
 467.28 649.78 254 58.53 0.932
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 201.0 6
0152-0655
 654.47 655.82 408 83.15 0.741
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 197.2 6
0153-0657
 227.78 657.24 307 67.36 0.850
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 182.2 6
0154-0663
 678.83 662.86 420 78.08 0.866