Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 2592.0 5
0005-0033
557.30 33.38 357 71.01 0.890
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 3634.8 5
0006-0035
647.06 35.19 464 81.50 0.878
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 3261.5 5
0007-0034
764.63 33.50 320 66.43 0.911
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 4640.2 5
0008-0038
17.47 38.56 167 48.28 0.900
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 957.1 5
0009-0038
843.73 38.50 257 60.18 0.892
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 3395.4 5
0010-0042
103.70 41.70 286 67.01 0.800
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1733.1 5
0011-0044
38.23 44.27 412 80.67 0.796
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 2716.1 5
0012-0040
618.67 39.46 201 53.11 0.895
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 2274.1 5
0013-0041
935.18 41.15 306 66.08 0.881
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 4771.5 5
0014-0045
205.15 45.67 328 68.18 0.887
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 998.0 5
0015-0046
353.28 45.99 285 63.36 0.892
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1506.7 5
0016-0046
438.62 46.09 260 60.77 0.885
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1599.7 5
0017-0047
119.53 46.85 227 64.53 0.685
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 5941.5 5
0018-0053
699.81 51.11 402 85.50 0.691
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 2187.6 5
0019-0054
728.32 54.59 354 69.26 0.927
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 5445.2 5
0020-0053
890.76 53.19 218 61.84 0.716
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 3641.4 5
0021-0058
502.59 57.05 187 55.01 0.776
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1498.0 5
0022-0061
125.53 61.72 186 57.60 0.705
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1837.0 5
0023-0061
392.17 61.28 300 68.77 0.797
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1671.6 5
0024-0063
865.42 63.69 284 63.36 0.889
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1607.1 5
0025-0063
445.32 62.52 211 55.11 0.873
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 3466.4 5
0026-0068
600.83 67.82 454 81.74 0.854
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 5835.1 5
0027-0065
641.80 65.14 294 62.77 0.938
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 10011.4 5
0028-0072
240.01 71.95 365 82.33 0.677
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 2383.0 5
0029-0078
458.41 79.37 349 74.67 0.787