Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 206.0 4
0100-0362
 531.38 361.51 870 114.57 0.833
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 191.1 4
0101-0364
 616.58 364.28 423 75.84 0.924
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 166.2 4
0102-0370
 180.75 369.39 448 80.67 0.865
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 197.2 4
0103-0375
 83.23 375.25 391 78.33 0.801
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 225.6 4
0104-0377
 425.81 377.11 425 78.91 0.858
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 219.7 4
0105-0386
 288.74 385.59 897 123.88 0.734
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 1786.9 4
0106-0384
 871.17 383.61 342 68.18 0.924
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 205.8 4
0107-0389
 24.18 389.00 376 74.67 0.847
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 211.4 4
0108-0387
 258.24 387.51 410 78.53 0.836
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 196.8 4
0109-0393
 491.32 393.10 437 83.15 0.794
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 200.4 4
0110-0396
 552.04 394.76 415 80.23 0.810
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 186.3 4
0111-0398
 738.64 397.49 397 74.43 0.901
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 241.8 4
0112-0399
 684.04 398.75 412 76.43 0.886
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 251.7 4
0113-0407
 389.42 406.76 529 94.91 0.738
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 176.0 4
0114-0407
 589.34 406.88 420 78.67 0.853
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 383.1 4
0115-0406
 920.59 406.58 440 77.26 0.926
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 273.7 4
0116-0413
 532.50 414.86 753 104.47 0.867
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 180.5 4
0117-0413
 233.92 412.73 412 77.26 0.867
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 219.9 4
0118-0411
 359.40 410.70 415 78.08 0.855
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 190.3 4
0119-0413
 630.06 411.74 381 77.64 0.794
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 186.9 4
0120-0419
 285.91 418.45 839 108.81 0.890
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 242.1 4
0121-0421
 695.14 420.42 479 90.81 0.730
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 233.1 4
0122-0419
 436.31 419.40 407 76.08 0.884
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 284.0 4
0123-0425
 110.14 425.64 562 94.81 0.786
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 219.4 4
0124-0426
 645.96 426.73 455 80.57 0.881