Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 248.2 8
0333-0540
 503.66 539.29 356 73.01 0.839
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 358.9 8
0334-0536
 837.33 536.46 168 51.11 0.808
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 227.0 8
0335-0540
 618.73 540.15 265 63.60 0.823
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 305.4 8
0336-0545
 108.17 545.82 246 60.77 0.837
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 46910.6 8
0337-0540
 310.74 540.38 112 45.11 0.692
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 268.7 8
0338-0544
 540.04 543.04 278 84.91 0.485
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 296.7 8
0339-0543
 647.59 542.83 260 59.94 0.909
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 310.6 8
0340-0543
 228.87 543.02 213 53.94 0.920
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 397.4 8
0341-0547
 440.74 547.05 393 74.18 0.897
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 521.7 8
0342-0547
 294.62 547.99 340 68.53 0.910
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 284.4 8
0343-0549
 63.74 548.50 494 85.84 0.842
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 237.6 8
0344-0547
 405.03 547.38 199 53.94 0.859
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 288.0 8
0345-0553
 720.38 553.08 424 78.91 0.856
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 242.8 8
0346-0555
 585.25 555.47 238 57.11 0.917
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 282.7 8
0347-0557
 930.45 557.56 203 53.94 0.877
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 400.3 8
0348-0561
 829.71 561.23 214 54.53 0.904
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 427.6 8
0349-0561
 149.59 561.09 177 51.11 0.851
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 279.0 8
0350-0565
 359.49 565.73 275 60.28 0.951
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 299.2 8
0351-0567
 899.27 567.38 262 59.60 0.927
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 390.3 8
0352-0583
 151.66 583.78 549 92.33 0.809
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 339.7 8
0353-0575
 301.99 574.27 211 54.53 0.892
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 281.2 8
0354-0579
 446.16 578.94 247 60.43 0.850
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 279.5 8
0355-0580
 653.80 579.80 251 60.77 0.854
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 282.4 8
0356-0578
 881.69 578.52 187 50.28 0.929
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 198.7 8
0357-0582
 591.28 583.00 268 61.26 0.898