Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 261.2 6
0201-0424
807.62 423.86 267 62.53 0.858
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 414.3 6
0202-0429
398.58 429.08 509 89.74 0.794
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 257.9 6
0203-0426
839.61 426.67 230 58.18 0.854
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 286.4 6
0204-0424
125.23 424.74 219 57.70 0.827
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 247.8 6
0205-0430
238.86 429.93 284 65.01 0.844
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 270.8 6
0206-0431
597.16 431.73 291 64.43 0.881
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 427.5 6
0207-0432
349.09 432.09 224 57.11 0.863
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 295.6 6
0208-0441
207.55 440.41 573 90.33 0.883
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 304.2 6
0209-0439
417.80 439.51 276 63.94 0.848
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 250.4 6
0210-0442
168.49 442.08 272 60.77 0.926
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 366.9 6
0211-0442
623.74 441.87 263 58.53 0.965
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 503.4 6
0212-0442
651.19 442.34 231 56.77 0.901
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 286.6 6
0213-0445
705.41 445.49 224 61.84 0.736
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 251.9 6
0214-0443
85.27 443.29 281 64.77 0.842
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 232.6 6
0215-0449
263.69 449.21 290 65.84 0.841
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 388.1 6
0216-0455
601.71 455.23 403 75.01 0.900
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 353.6 6
0217-0454
928.96 453.89 209 55.11 0.865
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 404.9 6
0218-0456
369.96 456.02 224 55.94 0.899
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 282.8 6
0219-0458
284.97 458.20 295 63.94 0.907
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 334.7 6
0220-0459
943.71 459.04 258 60.18 0.895
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 238.0 6
0221-0461
875.56 460.42 304 63.94 0.934
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 291.4 6
0222-0459
82.57 458.98 243 58.53 0.891
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 283.0 6
0223-0462
308.85 462.86 247 59.60 0.874
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 347.7 6
0224-0467
636.12 467.79 262 65.01 0.779
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 286.0 6
0225-0464
133.52 463.37 241 63.36 0.755