Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

 Full screen view
Filter results: >>>>
Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 329.1 4
0004-0069
 704.73 69.69 260 63.70 0.805
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 325.5 4
0005-0087
 675.87 87.02 277 61.70 0.914
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 381.9 4
0006-0092
 168.03 92.02 326 72.91 0.771
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 331.7 4
0007-0097
 488.16 97.07 264 62.53 0.849
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 445.0 4
0008-0106
 695.79 107.02 267 67.26 0.742
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 397.4 4
0009-0129
 134.25 130.00 186 56.87 0.723
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 450.0 4
0010-0144
 683.46 144.16 250 68.77 0.664
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 289.4 4
0011-0184
 270.60 185.09 156 47.46 0.870
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 318.4 4
0012-0184
 258.83 184.32 117 42.63 0.809
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 305.9 4
0013-0198
 252.47 198.14 271 61.60 0.898
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 301.8 4
0014-0202
 271.16 202.46 228 62.77 0.727
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 398.3 4
0015-0222
 683.17 222.21 211 63.60 0.656
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 313.0 4
0016-0223
 626.05 223.64 268 61.60 0.888
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 303.9 4
0017-0230
 296.16 230.32 363 80.67 0.701
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 321.0 4
0018-0236
 870.80 235.72 251 60.53 0.861
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 807.6 4
0019-0248
 297.88 248.35 171 67.84 0.467
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 317.0 4
0020-0251
 61.22 251.90 222 54.77 0.930
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 270.0 4
0021-0252
 929.07 252.31 258 60.77 0.878
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 295.4 4
0022-0261
 757.81 261.64 281 65.01 0.835
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 333.1 4
0023-0260
 858.20 260.13 218 61.70 0.720
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 333.9 4
0024-0273
 154.52 273.23 239 64.53 0.721
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 271.3 4
0025-0274
 848.11 274.54 288 65.94 0.832
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 456.9 4
0026-0278
 57.57 278.85 196 52.53 0.893
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 358.2 4
0027-0290
 821.95 289.82 232 58.53 0.851
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 265.3 4
0028-0297
 30.71 296.88 302 66.77 0.851