Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

 Full screen view
Filter results: >>>>
Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 386.5 3
0037-0402
 50.40 402.63 241 60.77 0.820
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 431.9 3
0038-0407
 742.68 408.20 266 64.18 0.811
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 307.5 3
0039-0408
 893.26 407.85 285 67.26 0.792
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 436.8 3
0040-0454
 910.50 453.38 237 58.77 0.862
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 294.7 3
0041-0486
 783.43 486.83 257 62.18 0.835
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 483.1 3
0042-0489
 453.81 489.03 234 57.36 0.894
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 384.3 3
0043-0499
 369.03 498.82 247 60.18 0.857
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 328.8 3
0044-0535
 663.85 535.40 257 62.87 0.817
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 376.1 3
0045-0539
 343.31 539.41 471 89.74 0.735
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 551.8 3
0046-0531
 785.86 531.44 177 51.11 0.851
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 472.6 3
0047-0549
 945.50 548.90 266 68.63 0.710
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 320.8 3
0048-0558
 808.51 557.74 322 68.18 0.870
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 653.1 3
0049-0560
 315.90 559.69 220 93.40 0.317
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 318.3 3
0050-0565
 444.93 564.88 283 66.18 0.812
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 316.1 3
0051-0575
 624.95 575.22 373 72.18 0.900
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 306.5 3
0052-0581
 325.62 582.28 262 61.94 0.858
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 320.8 3
0053-0609
 835.51 610.13 302 66.18 0.866
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 522.4 3
0054-0645
 276.97 644.96 208 56.87 0.808
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 289.8 3
0055-0663
 699.65 662.76 264 65.36 0.777
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 436.5 3
0056-0664
 832.78 663.96 216 59.84 0.758
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 536.9 3
0057-0666
 97.42 666.26 212 53.94 0.916
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 317.2 3
0058-0670
 618.50 669.36 259 66.43 0.738
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 421.7 4
0001-0035
 57.39 34.95 202 56.77 0.788
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 371.9 4
0002-0036
 140.39 36.89 263 67.26 0.731
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 262.6 4
0003-0065
 897.16 64.19 268 63.26 0.842