Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 323.3 5
0029-0153
739.43 153.21 377 71.01 0.939
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 323.3 5
0030-0155
266.99 155.23 390 74.08 0.893
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 350.4 5
0031-0158
364.21 158.24 390 74.43 0.885
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 314.9 5
0032-0157
789.19 157.46 339 69.84 0.873
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 477.1 5
0033-0161
428.45 161.27 415 79.15 0.832
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 366.1 5
0034-0162
606.91 162.23 471 85.98 0.801
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 254.2 5
0035-0164
102.21 164.37 385 74.91 0.862
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 315.6 5
0036-0170
393.74 169.85 402 75.84 0.878
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 412.7 5
0037-0173
45.29 173.71 724 105.50 0.817
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 520.9 5
0038-0176
559.97 176.57 534 125.44 0.426
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 254.5 5
0039-0182
182.79 182.45 382 74.33 0.869
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 274.1 5
0040-0184
824.00 184.55 431 79.84 0.850
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 238.6 5
0041-0189
630.02 190.57 822 113.54 0.801
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 253.2 5
0042-0185
228.36 183.91 443 87.05 0.735
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 364.9 5
0043-0202
452.20 201.93 838 110.57 0.861
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 400.5 5
0044-0195
279.94 195.23 410 77.01 0.869
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 377.3 5
0045-0202
155.62 202.56 473 86.08 0.802
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 323.6 5
0046-0201
527.69 201.62 461 81.84 0.865
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 260.1 5
0047-0203
752.82 203.24 401 76.08 0.871
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 310.5 5
0048-0210
326.39 209.37 856 119.20 0.757
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 221.8 5
0049-0207
228.68 207.78 408 82.23 0.758
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 222.7 5
0050-0218
126.09 217.93 392 77.74 0.815
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 193.4 5
0051-0219
938.78 219.02 391 74.43 0.887
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 229.7 5
0052-0223
167.61 222.46 356 76.43 0.766
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 326.5 5
0053-0229
91.30 229.29 380 74.67 0.856