Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 243.2 5
0004-0043
672.96 42.74 427 77.84 0.886
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 216.0 5
0005-0047
55.66 46.50 612 93.98 0.871
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 254.0 5
0006-0047
87.28 46.68 387 97.15 0.515
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 271.9 5
0007-0044
186.17 44.47 395 73.84 0.910
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 274.3 5
0008-0047
226.42 47.21 458 83.15 0.832
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 360.3 5
0009-0048
129.84 47.85 425 77.01 0.900
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 348.4 5
0010-0050
790.39 50.36 408 77.74 0.848
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 153.8 5
0011-0051
101.71 52.26 139 54.53 0.587
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 284.8 5
0012-0057
312.17 57.19 430 79.26 0.860
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 282.2 5
0013-0068
489.97 68.32 441 79.50 0.877
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 296.9 5
0014-0073
372.57 73.36 407 74.43 0.923
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 308.9 5
0015-0074
412.97 74.21 406 77.50 0.849
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 272.0 5
0016-0082
710.92 82.13 436 77.01 0.924
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 320.3 5
0017-0089
155.98 89.08 370 72.43 0.886
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 288.5 5
0018-0098
744.01 98.60 365 71.26 0.903
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 325.2 5
0019-0101
112.51 100.66 349 70.43 0.884
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 260.8 5
0020-0107
189.04 107.38 386 77.50 0.808
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 419.5 5
0021-0106
912.13 105.94 263 75.50 0.580
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 253.7 5
0022-0110
20.05 109.88 419 79.74 0.828
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 282.9 5
0023-0113
684.55 113.61 430 78.08 0.886
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 672.7 5
0024-0134
634.63 132.56 822 126.37 0.647
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 238.5 5
0025-0135
217.50 135.58 433 79.84 0.854
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 274.4 5
0026-0138
151.19 137.96 392 74.67 0.884
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 199.8 5
0027-0139
694.30 139.48 432 78.91 0.872
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 447.9 5
0028-0138
506.42 138.97 366 71.26 0.906