Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 453.5 4
0002-0030
 469.53 29.71 401 77.50 0.839
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 262.6 4
0003-0032
 326.73 31.62 416 76.67 0.889
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 286.3 4
0004-0035
 166.80 35.50 385 74.67 0.868
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 267.3 4
0005-0034
 539.01 34.48 445 82.43 0.823
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 335.1 4
0006-0034
 38.45 34.57 375 74.33 0.853
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 347.4 4
0007-0036
 206.75 36.76 415 80.57 0.803
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 244.4 4
0008-0042
 639.40 41.71 375 73.50 0.872
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 249.5 4
0009-0047
 481.05 47.22 481 85.98 0.818
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 375.0 4
0010-0051
 403.40 51.21 468 82.33 0.868
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 222.7 4
0011-0061
 606.13 61.23 465 82.08 0.867
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 233.9 4
0012-0061
 870.01 62.05 423 76.67 0.904
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 307.2 4
0013-0064
 814.30 64.40 384 72.77 0.911
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 310.7 4
0014-0070
 776.89 69.91 432 80.08 0.846
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 273.3 4
0015-0069
 362.99 69.22 421 80.33 0.820
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 225.6 4
0016-0075
 690.51 75.10 436 78.43 0.891
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 310.0 4
0017-0076
 236.77 76.65 410 77.50 0.858
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 262.1 4
0018-0077
 538.56 77.41 475 87.15 0.786
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 298.2 4
0019-0084
 839.75 83.66 374 72.77 0.888
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 202.4 4
0020-0087
 511.66 87.27 484 87.15 0.801
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 278.0 4
0021-0100
 67.65 99.83 411 77.26 0.865
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 277.0 4
0022-0102
 191.26 101.85 442 80.67 0.854
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 288.3 4
0023-0106
 411.29 105.96 766 104.91 0.875
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 380.8 4
0024-0103
 436.90 103.15 459 81.36 0.871
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 236.4 4
0025-0103
 24.48 103.70 398 75.84 0.870
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 258.2 4
0026-0108
 564.96 108.42 928 128.95 0.701