Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 622.3 5
0036-0419
 429.87 419.08 156 58.77 0.568
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 700.5 5
0037-0425
 50.46 424.75 261 63.60 0.811
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 219.5 5
0038-0484
 95.58 484.30 269 62.18 0.874
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 582.5 5
0039-0483
 930.96 483.42 201 53.70 0.876
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 424.9 5
0040-0494
 633.21 493.73 212 62.28 0.687
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2360.3 5
0041-0497
 769.80 497.55 253 61.70 0.835
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 756.5 5
0042-0508
 329.53 508.48 329 72.43 0.788
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2690.6 5
0043-0527
 286.51 526.75 220 61.01 0.743
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 541.4 5
0044-0535
 622.69 534.63 187 56.28 0.742
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 239.5 5
0045-0550
 220.89 550.54 202 57.94 0.756
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 265.6 5
0046-0548
 249.45 548.43 172 52.28 0.791
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 430.6 5
0047-0563
 153.70 562.95 110 53.36 0.486
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 676.1 5
0048-0583
 634.62 583.75 264 63.84 0.814
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 3019.3 5
0049-0594
 129.69 594.89 191 54.18 0.818
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1483.8 5
0050-0606
 526.68 606.72 254 62.53 0.816
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 439.0 5
0051-0621
 548.39 620.38 261 60.53 0.895
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 3306.8 5
0052-0633
 394.79 633.43 175 57.94 0.655
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 201.1 5
0053-0649
 879.38 648.46 267 62.77 0.852
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 884.8 5
0054-0658
 86.40 658.34 208 66.04 0.599
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1489.1 5
0055-0659
 146.43 658.61 215 57.11 0.828
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1378.8 5
0056-0676
 660.44 675.54 242 63.36 0.758
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 287.1 5
0057-0680
 392.72 679.46 237 61.94 0.776
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2287.2 5
0058-0686
 413.76 685.86 186 51.70 0.875
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2324.7 5
0059-0701
 377.39 701.40 197 55.70 0.798
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 302.2 5
0060-0703
 482.30 702.70 182 64.67 0.547