Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 1977.5 6
0289-0505
 333.16 504.68 199 54.87 0.831
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 4330.7 6
0290-0509
 934.43 509.12 471 88.57 0.755
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 758.7 6
0291-0510
 601.38 510.61 350 70.43 0.887
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 2606.5 6
0292-0512
 911.09 511.98 298 68.18 0.805
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 1530.8 6
0293-0514
 516.97 514.15 236 57.70 0.891
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 1289.3 6
0294-0515
 42.43 515.91 283 62.18 0.920
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 1372.0 6
0295-0516
 653.99 516.16 302 66.18 0.866
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 1227.0 6
0296-0518
 896.99 517.89 228 60.77 0.776
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 4036.4 6
0297-0518
 64.81 518.39 236 56.77 0.920
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 1093.1 6
0298-0521
 259.18 521.20 288 64.43 0.872
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 2260.6 6
0299-0522
 759.26 522.01 321 66.77 0.905
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 1027.9 6
0300-0521
 695.19 521.83 316 67.84 0.863
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 1114.3 6
0301-0524
 307.39 524.49 237 63.60 0.736
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 2971.1 6
0302-0524
 537.36 523.04 202 57.36 0.772
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 3229.7 6
0303-0526
 738.26 525.71 335 67.11 0.935
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 2737.9 6
0304-0530
 442.40 529.89 215 53.94 0.929
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 1617.6 6
0305-0529
 222.70 529.77 259 62.77 0.826
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 1409.1 6
0306-0533
 148.88 533.45 266 64.77 0.797
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 1953.9 6
0307-0537
 520.57 536.09 307 66.18 0.881
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 835.6 6
0308-0540
 795.24 540.18 237 62.77 0.756
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 898.1 6
0309-0541
 927.11 540.64 263 61.36 0.878
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 1672.2 6
0310-0541
 111.28 540.83 251 61.94 0.822
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 3387.3 6
0311-0538
 356.39 538.08 144 45.36 0.880
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 2178.0 6
0312-0542
 430.57 542.86 238 62.43 0.767
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 1991.4 6
0313-0544
 37.62 545.15 245 60.18 0.850